Dyadyusha H P, Sanina O L
Ukr Biokhim Zh. 1975 Mar-Apr;47(2):172-8.
It is shown that simultaneous treatment with sarcolemma with triton X-100 (0.1-1.0%) and KCl (0.6-1.2 M) solubilizes about 60% of acetyl cholinesterase, however its specific activity in the intact sarcolemma and in the obtained fractions is the same. A considerable part of lipids (about 90% of phospholipids and 50% of cholesterol) may be removed by treating sarcolemma with 0.1% triton X-100; it may be fixed in the membrane by hydrophoric bonds. Other phospholipids and cholesterol are bound with sarcolemma proteins more strongly and, probably, form the protein-lipid complexes. In the composition of phospholipids strongly bound with sarcolemma proteins a relative content of sphingomyelin increases and that of lysolecithin decreases.
结果表明，用曲拉通X - 100（0.1 - 1.0%）和氯化钾（0.6 - 1.2 M）同时处理肌膜，可使约60%的乙酰胆碱酯酶溶解，但其在完整肌膜和所得组分中的比活性相同。用0.1%曲拉通X - 100处理肌膜可去除相当一部分脂质（约90%的磷脂和50%的胆固醇）；它可能通过疏水键固定在膜中。其他磷脂和胆固醇与肌膜蛋白结合更紧密，可能形成蛋白质 - 脂质复合物。在与肌膜蛋白紧密结合的磷脂组成中，鞘磷脂的相对含量增加，溶血卵磷脂的相对含量降低。
