卡波西肉瘤相关疱疹病毒（人类疱疹病毒8型）含有两个功能性DNA复制裂解起始位点。
Kaposi's sarcoma-associated herpesvirus (human herpesvirus 8) contains two functional lytic origins of DNA replication.
作者信息
AuCoin David P, Colletti Kelly S, Xu Yiyang, Cei Sylvia A, Pari Gregory S
机构信息
Department of Microbiology and Cell and Molecular Biology Program, University of Nevada School of Medicine, Reno, Nevada 89557, USA.
出版信息
J Virol. 2002 Aug;76(15):7890-6. doi: 10.1128/jvi.76.15.7890-7896.2002.
Abstract
We used a transient-transfection replication assay to identify two functional copies of the human herpesvirus 8 (HHV8) lytic origin of DNA replication (oriLyt). BCLB-1 cells were transfected with HHV8 subgenomic fragments containing the putative lytic origin along with a plasmid expressing viral transactivator open reading frame (ORF) 50. The HHV8 left-end oriLyt (oriLyt-L) lies between ORFs K4.2 and K5 and is composed of a region encoding various transcription factor binding sites and an A+T-rich region and a G+C repeat region. The right-end oriLyt (oriLyt-R) maps between ORF 69 and vFLIP, a region similar to the RRV oriLyt, and is an inverted duplication of oriLyt-L.
摘要
我们使用瞬时转染复制分析来鉴定人类疱疹病毒8（HHV8）DNA复制裂解起始点（oriLyt）的两个功能拷贝。将含有假定裂解起始点的HHV8亚基因组片段与表达病毒反式激活因子开放阅读框（ORF）50的质粒一起转染到BCLB - 1细胞中。HHV8左端oriLyt（oriLyt - L）位于ORF K4.2和K5之间，由一个编码各种转录因子结合位点的区域、一个富含A + T的区域和一个G + C重复区域组成。右端oriLyt（oriLyt - R）定位在ORF 69和vFLIP之间，该区域与RRV oriLyt相似，是oriLyt - L的反向重复序列。
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