Sun R, Lin S F, Gradoville L, Yuan Y, Zhu F, Miller G
Departments of Molecular Biophysics and Biochemistry, Genetics, Pediatrics, and Epidemiology and Public Health, Yale University School of Medicine, New Haven, CT 06520, USA.
Proc Natl Acad Sci U S A. 1998 Sep 1;95(18):10866-71. doi: 10.1073/pnas.95.18.10866.
Herpesviruses exist in two states, latency and a lytic productive cycle. Here we identify an immediate-early gene encoded by Kaposi's sarcoma-associated herpesvirus (KSHV)/human herpesvirus eight (HHV8) that activates lytic cycle gene expression from the latent viral genome. The gene is a homologue of Rta, a transcriptional activator encoded by Epstein-Barr virus (EBV). KSHV/Rta activated KSHV early lytic genes, including virus-encoded interleukin 6 and polyadenylated nuclear RNA, and a late gene, small viral capsid antigen. In cells dually infected with Epstein-Barr virus and KSHV, each Rta activated only autologous lytic cycle genes. Expression of viral cytokines under control of the KSHV/Rta gene is likely to contribute to the pathogenesis of KSHV-associated diseases.
疱疹病毒以两种状态存在，即潜伏状态和裂解性增殖周期。在此我们鉴定出一种由卡波西肉瘤相关疱疹病毒（KSHV）/人疱疹病毒8型（HHV8）编码的立即早期基因，该基因可激活潜伏病毒基因组中的裂解周期基因表达。该基因是Rta的同源物，Rta是一种由爱泼斯坦-巴尔病毒（EBV）编码的转录激活因子。KSHV/Rta激活了KSHV早期裂解基因，包括病毒编码的白细胞介素6和多聚腺苷酸化核RNA，以及一个晚期基因，小病毒衣壳抗原。在同时感染爱泼斯坦-巴尔病毒和KSHV的细胞中，每种Rta仅激活自身的裂解周期基因。在KSHV/Rta基因控制下病毒细胞因子的表达可能有助于KSHV相关疾病的发病机制。
