Boussery Koen, Franki Ann Sophie, Delaey Christophe, Van de Voorde Johan
Department of Physiology and Pathophysiology, Ghent University, De Pintelaan 185, Blok B, B-9000 Ghent, Belgium.
Ophthalmic Res. 2002 May-Jun;34(3):172-7. doi: 10.1159/000063662.
The present study aimed to demonstrate the release of a retinal relaxing factor (RRF) from the retina of mice and to investigate the identity of the RRF. Ring segments of a mouse aorta were mounted in a small vessel myograph. The relaxing influence of mouse retinal tissue was assessed by placing a retina in close proximity to the precontracted aorta. This elicited reliable and reproducible relaxations in the aorta. Both the nitric oxide (NO) synthase inhibitor N(omega)-nitro-L-arginine and the soluble guanylyl cyclase inhibitor 1H-(1,2,4)oxadiazolo(4,3-a)quinoxalin-1-one had no effect on the RRF response. Also the cyclooxygenase inhibitors indomethacin and sodium diclofenac failed to affect the retina-induced relaxations. Acute hypoxia largely enhanced retina-induced relaxations. It is concluded that mouse retinal tissue releases an RRF, that the mouse RRF response is not mediated by NO or prostanoids and that the mouse RRF response is profoundly influenced by hypoxia.
本研究旨在证明小鼠视网膜释放一种视网膜舒张因子（RRF），并探究RRF的特性。将小鼠主动脉的环形节段安装在小型血管肌动描记器中。通过将视网膜与预收缩的主动脉紧密相邻放置来评估小鼠视网膜组织的舒张作用。这在主动脉中引发了可靠且可重复的舒张。一氧化氮（NO）合酶抑制剂N（ω）-硝基-L-精氨酸和可溶性鸟苷酸环化酶抑制剂1H-（1,2,4）恶二唑并（4,3-a）喹喔啉-1-酮对RRF反应均无影响。环氧化酶抑制剂吲哚美辛和双氯芬酸钠也未能影响视网膜诱导的舒张。急性缺氧在很大程度上增强了视网膜诱导的舒张。得出的结论是，小鼠视网膜组织释放一种RRF，小鼠RRF反应不是由NO或前列腺素介导的，并且小鼠RRF反应受到缺氧的深刻影响。
