盘基网柄菌2号染色体的序列与分析

Sequence and analysis of chromosome 2 of Dictyostelium discoideum.

作者信息

Glöckner Gernot, Eichinger Ludwig, Szafranski Karol, Pachebat Justin A, Bankier Alan T, Dear Paul H, Lehmann Rüdiger, Baumgart Cornelia, Parra Genís, Abril Josep F, Guigó Roderic, Kumpf Kai, Tunggal Budi, Cox Edward, Quail Michael A, Platzer Matthias, Rosenthal André, Noegel Angelika A

机构信息

IMB Jena, Department of Genome Analysis, Beutenbergstr. 11, 07745 Jena, Germany.

出版信息

Nature. 2002 Jul 4;418(6893):79-85. doi: 10.1038/nature00847.

DOI:10.1038/nature00847

PMID:12097910

Abstract

The genome of the lower eukaryote Dictyostelium discoideum comprises six chromosomes. Here we report the sequence of the largest, chromosome 2, which at 8 megabases (Mb) represents about 25% of the genome. Despite an A + T content of nearly 80%, the chromosome codes for 2,799 predicted protein coding genes and 73 transfer RNA genes. This gene density, about 1 gene per 2.6 kilobases (kb), is surpassed only by Saccharomyces cerevisiae (one per 2 kb) and is similar to that of Schizosaccharomyces pombe (one per 2.5 kb). If we assume that the other chromosomes have a similar gene density, we can expect around 11,000 genes in the D. discoideum genome. A significant number of the genes show higher similarities to genes of vertebrates than to those of other fully sequenced eukaryotes. This analysis strengthens the view that the evolutionary position of D. discoideum is located before the branching of metazoa and fungi but after the divergence of the plant kingdom, placing it close to the base of metazoan evolution.

摘要

低等真核生物盘基网柄菌的基因组由6条染色体组成。在此，我们报告其最大的染色体2的序列，该染色体大小为8兆碱基（Mb），约占基因组的25%。尽管其A + T含量接近80%，但该染色体编码2799个预测的蛋白质编码基因和73个转运RNA基因。这种基因密度约为每2.6千碱基（kb）有1个基因，仅次于酿酒酵母（每2 kb有1个基因），与粟酒裂殖酵母（每2.5 kb有1个基因）相似。如果我们假设其他染色体具有相似的基因密度，那么盘基网柄菌基因组中大约有11000个基因。相当数量的基因与脊椎动物的基因相似度高于与其他已完成全序列测序的真核生物的基因相似度。这一分析强化了这样一种观点，即盘基网柄菌的进化位置处于后生动物和真菌分支之前，但在植物界分化之后，使其靠近后生动物进化的基部。