Djebaïli Myriam, De Bock Frédéric, Baille Valérie, Bockaert Joël, Rondouin Gérard
UPR CNRS 9023, CCIPE, Laboratoire de Médecine Expérimentale, Institut de Biologie, Boulevard Henri IV, 34094 Montpellier Cedex 5, France.
Neurosci Lett. 2002 Jul 12;327(1):1-4. doi: 10.1016/s0304-3940(02)00137-4.
Apoptotic death is known to be an active process requiring the activation of several apoptotic proteins. Depending on the tissue studied and the stimulus used, these processes are distinct. In this work, we studied if there is a putative implication of the p53 and the caspase-3 proteins in kainic acid (KA) and N-methyl-D-aspartic acid (NMDA)-induced apoptosis in organotypic cultures and if there is any relationship between their respective expressions. We found that KA and NMDA both induce apoptosis but only KA-induced apoptosis is p53- and caspase-3-dependent. This demonstrates that KA and NMDA induce apoptosis following different intracellular pathways.
已知凋亡性死亡是一个需要激活多种凋亡蛋白的活跃过程。根据所研究的组织和所使用的刺激因素，这些过程是不同的。在这项工作中，我们研究了在器官型培养物中，p53和半胱天冬酶-3蛋白是否可能参与了海藻酸（KA）和N-甲基-D-天冬氨酸（NMDA）诱导的凋亡，以及它们各自的表达之间是否存在任何关系。我们发现，KA和NMDA都能诱导凋亡，但只有KA诱导的凋亡依赖于p53和半胱天冬酶-3。这表明KA和NMDA通过不同的细胞内途径诱导凋亡。
