Xu Kan-Yan, Xiong Ying, Du Yu-Cang
Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, the Chinese Academy of Sciences, Shanghai 200031, China. duyc@ sunm.shnc.ac.cn
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 2002 Jul;34(4):418-22.
In order to study the effect of argipressin(4-8)(AVP(4-8)) on the mRNA level and activity of cytidine triphosphate: phosphocholine cytidylyltransferase(CCT) in rat hippocampal neurons, and elucidate it's possible mechanism. Rat hippocampal neurons treated with AVP(4-8) or actinomycin D were incubated with different time periods. The mRNA level of CCT was detected using RT-PCR plus Southern blot, CCT activity was determined by measuring the rate of incorporation of (14)C - phosphocholine into cytidine diphosphate-choline(CDP-choline). It was found that AVP4-8 could upregulate the CCT mRNA in rat hippocampal neurons. ZDC(C)PR, the antagonist of AVP(4-8), could greatly inhibit this upregulation. Using actinomycin D to inhibite the eucaryotic transcription, it was found that the halflife of CCT mRNA could be prolonged by coincubation with AVP(4-8). Meanwhile, AVP(4-8) could also increase CCT activity in rat hippocampal neurons. These results demonstrated that AVP(4-8) upregulated CCT mRNA level and its activity through stabilizing the CCT mRNA in rat hippocampal neurons.
为研究精氨酸加压素（4 - 8）（AVP（4 - 8））对大鼠海马神经元中三磷酸胞苷：磷酸胆碱胞苷转移酶（CCT）的mRNA水平及活性的影响，并阐明其可能机制。用AVP（4 - 8）或放线菌素D处理大鼠海马神经元，并孵育不同时间段。采用逆转录聚合酶链反应（RT - PCR）加Southern印迹法检测CCT的mRNA水平，通过测定（14）C - 磷酸胆碱掺入二磷酸胞苷胆碱（CDP - 胆碱）的速率来确定CCT活性。结果发现，AVP4 - 8可上调大鼠海马神经元中的CCT mRNA。AVP（4 - 8）的拮抗剂ZDC（C）PR可显著抑制这种上调。用放线菌素D抑制真核转录后发现，与AVP（4 - 8）共同孵育可延长CCT mRNA的半衰期。同时，AVP（4 - 8）还可增加大鼠海马神经元中的CCT活性。这些结果表明，AVP（4 - 8）通过稳定大鼠海马神经元中的CCT mRNA上调其mRNA水平及活性。
