Wright J Barry, Lam Kan, Buret Andre G, Olson Merle E, Burrell Robert E
Westaim Biomedical Corporation, Fort Saskatchewan, Canada.
Wound Repair Regen. 2002 May-Jun;10(3):141-51. doi: 10.1046/j.1524-475x.2002.10308.x.
A porcine model of wound healing was employed to examine the impact of nanocrystalline silver-coated dressings on specific wound healing events. Full-thickness wounds were created on the backs of pigs, contaminated with an experimental inoculum containing Pseudomonas aeruginosa, Fusobacterium sp., and coagulase-negative staphylococci, and covered with dressing products either containing silver or not. Nanocrystalline silver-coated dressings promoted rapid wound healing, particularly during the first several days post-injury. Healing was characterized by rapid development of well vascularized granulation tissue that supported tissue grafting 4 days post-injury, unlike control dressed wounds. The proteolytic environment of wounds treated with nanocrystalline silver was characterized by reduced levels of matrix metalloproteinases. Matrix metalloproteinases have been shown to be present in chronic ulcers at abnormally high levels, as compared with acute wounds, and may contribute to the nonhealing nature of these wounds. Cellular apoptosis occurred at a higher frequency in the nanocrystalline silver-treated wounds than in wounds dressed with other products. The results suggest that nanocrystalline silver may play a role in altering or compressing the inflammatory events in wounds and facilitating the early phases of wound healing. These benefits are associated with reduced local matrix metalloproteinase levels and enhanced cellular apoptosis.
采用猪伤口愈合模型来研究纳米晶银涂层敷料对特定伤口愈合过程的影响。在猪的背部制造全层伤口，用含有铜绿假单胞菌、梭杆菌属和凝固酶阴性葡萄球菌的实验接种物污染伤口，并用含银或不含银的敷料产品覆盖。纳米晶银涂层敷料促进伤口快速愈合，尤其是在受伤后的头几天。与对照包扎伤口不同，愈合的特征是在受伤4天后迅速形成血管丰富的肉芽组织，支持组织移植。用纳米晶银处理的伤口的蛋白水解环境的特征是基质金属蛋白酶水平降低。与急性伤口相比，基质金属蛋白酶已被证明在慢性溃疡中异常高水平存在，并且可能导致这些伤口不愈合。纳米晶银处理的伤口中细胞凋亡的频率高于用其他产品包扎的伤口。结果表明，纳米晶银可能在改变或压缩伤口中的炎症事件以及促进伤口愈合的早期阶段发挥作用。这些益处与局部基质金属蛋白酶水平降低和细胞凋亡增强有关。
