Zhang Li, Schallert Timothy, Zhang Zheng Gang, Jiang Quan, Arniego Polly, Li Qingjiang, Lu Mei, Chopp Michael
Department of Neurology, Henry Ford Hospital, 2799 West Grand Boulevard, Detroit, MI 48202, USA.
J Neurosci Methods. 2002 Jun 30;117(2):207-14. doi: 10.1016/s0165-0270(02)00114-0.
The mouse is an excellent model for investigations of stroke and neural injury. However, there is a paucity of long term functional outcome measurements for the mouse. We, therefore, developed a sensorimotor functional test (corner test) and applied this test to a model of focal cerebral ischemia in the mouse. Male C57/6J mice (n=20) were subjected to embolic middle cerebral artery (MCA) occlusion. Reduction of cerebral blood flow (CBF) was measured by perfusion weighted MRI at 1 h after ischemia. The corner test, which is sensitive to chronic sensorimotor and postural symmetries, a general neurological test battery, and a foot fault test were performed between 2 and 90 days after ischemia. Infarct volume was measured at 90 days after ischemia. Multivariable analysis revealed that the corner test was highly predictive for infarct volume measured at 90 days after stroke, with R(2) values ranging from 0.73 to 0.93. The foot-fault test and neurological score did not detect chronic behavioral impairments. A significant (P<0.001) correlation between the infarct volume and the corner test was detected at 90 days after mild focal cerebral ischemia, whereas, there was no correlation between the infarct volume and neurological score or foot-fault. The data demonstrate that the corner test is a sensitive and objective test, which can be applied to evaluate long term functional outcome after stroke in the mouse.
小鼠是研究中风和神经损伤的优秀模型。然而，针对小鼠的长期功能结局测量方法却很匮乏。因此，我们开发了一种感觉运动功能测试（角落测试），并将该测试应用于小鼠局灶性脑缺血模型。雄性C57/6J小鼠（n = 20）接受栓塞性大脑中动脉（MCA）闭塞手术。缺血1小时后通过灌注加权MRI测量脑血流量（CBF）减少情况。在缺血后2至90天进行角落测试（该测试对慢性感觉运动和姿势对称性敏感）、一般神经学测试组以及足部失误测试。在缺血90天后测量梗死体积。多变量分析显示，角落测试对中风后90天测量的梗死体积具有高度预测性，R(2)值范围为0.73至0.93。足部失误测试和神经学评分未检测到慢性行为损伤。在轻度局灶性脑缺血90天后，梗死体积与角落测试之间检测到显著（P<0.001）相关性，而梗死体积与神经学评分或足部失误之间无相关性。数据表明，角落测试是一种敏感且客观的测试，可用于评估小鼠中风后的长期功能结局。
