Hamann Thorsten, Benkova Eva, Bäurle Isabel, Kientz Marika, Jürgens Gerd
ZMBP, Entwicklungsgenetik, Universität Tübingen, D-72076 Tübingen, Federal Republic of Germany.
Genes Dev. 2002 Jul 1;16(13):1610-5. doi: 10.1101/gad.229402.
Developmental responses to the plant hormone auxin are thought to be mediated by interacting pairs from two protein families: short-lived inhibitory IAA proteins and ARF transcription factors binding to auxin-response elements. monopteros mutants lacking activating ARF5 and the auxin-insensitive mutant bodenlos fail to initiate the root meristem during early embryogenesis. Here we show that the bodenlos phenotype results from an amino-acid exchange in the conserved degradation domain of IAA12. BODENLOS and MONOPTEROS interact in the yeast two-hybrid assay and the two genes are coexpressed in early embryogenesis, suggesting that BODENLOS inhibits MONOPTEROS action in root meristem initiation.
短命的抑制性IAA蛋白和与生长素反应元件结合的ARF转录因子。缺乏激活型ARF5的单翅目突变体和生长素不敏感突变体无柄在早期胚胎发生过程中无法启动根分生组织。我们在此表明,无柄表型是由IAA12保守降解结构域中的氨基酸交换导致的。在酵母双杂交试验中,无柄和单翅目相互作用,并且这两个基因在早期胚胎发生中共同表达,这表明无柄在根分生组织起始中抑制单翅目的作用。