松科(Pinus koraiensis)胚胎发育过程中 ARF 基因家族的全基因组鉴定和表达分析。

Genome-wide identification and expression analysis of ARF gene family in embryonic development of Korean pine (Pinus koraiensis).

机构信息

State Key Laboratory of Tree Genetics and Breeding, College of Forestry, Northeast Forestry University, Harbin, 150040, China.

State Forestry and Grassland Administration Engineering Technology Research Center of Korean Pine, Harbin, 150040, China.

出版信息

BMC Plant Biol. 2024 Apr 10;24(1):267. doi: 10.1186/s12870-024-04827-w.

DOI:10.1186/s12870-024-04827-w

PMID:38600459

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11005186/

Abstract

BACKGROUND

The Auxin Responsive Factor (ARF) family plays a crucial role in mediating auxin signal transduction and is vital for plant growth and development. However, the function of ARF genes in Korean pine (Pinus koraiensis), a conifer species of significant economic value, remains unclear.

RESULTS

This study utilized the whole genome of Korean pine to conduct bioinformatics analysis, resulting in the identification of 13 ARF genes. A phylogenetic analysis revealed that these 13 PkorARF genes can be classified into 4 subfamilies, indicating the presence of conserved structural characteristics within each subfamily. Protein interaction prediction indicated that Pkor01G00962.1 and Pkor07G00704.1 may have a significant role in regulating plant growth and development as core components of the PkorARFs family. Additionally, the analysis of RNA-seq and RT-qPCR expression patterns suggested that PkorARF genes play a crucial role in the development process of Korean pine.

CONCLUSION

Pkor01G00962.1 and Pkor07G00704.1, which are core genes of the PkorARFs family, play a potentially crucial role in regulating the fertilization and developmental process of Korean pine. This study provides a valuable reference for investigating the molecular mechanism of embryonic development in Korean pine and establishes a foundation for cultivating high-quality Korean pine.

摘要

背景

生长素响应因子（ARF）家族在介导生长素信号转导中起着至关重要的作用，对植物的生长和发育至关重要。然而，ARF 基因在具有重要经济价值的针叶树种红松（Pinus koraiensis）中的功能尚不清楚。

结果

本研究利用红松的全基因组进行生物信息学分析，鉴定出 13 个 ARF 基因。系统发育分析表明，这 13 个 PkorARF 基因可以分为 4 个亚家族，表明每个亚家族都具有保守的结构特征。蛋白质相互作用预测表明，Pkor01G00962.1 和 Pkor07G00704.1 可能作为 PkorARFs 家族的核心组成部分，在调节植物生长和发育方面发挥重要作用。此外，RNA-seq 和 RT-qPCR 表达模式分析表明，PkorARF 基因在红松发育过程中发挥着重要作用。