Uhl E W, Giguère S, Jack T J, Hodge T
Department of Pathobiology, College of Veterinary Medicine, University of Florida, Gainesville 32610-0145, USA.
Vet Pathol. 2002 Jan;39(1):132-6. doi: 10.1354/vp.39-1-132.
Previous studies revealed that foals inoculated with virulent Rhodococcus equi had significantly higher pulmonary levels of interleukin-1beta, interleukin-12 p40, interferon-gamma, and tumor necrosis factor-alpha mRNA compared to foals inoculated with an avirulent plasmid-cured derivative. The purpose of this study was to determine if the increases in cytokine expression were associated with increased pulmonary activation of nuclear factor-kappaB (NF-kappaB). Electrophoretic mobility shift assays were performed on pulmonary nuclear protein extracted from foals treated with phosphate-buffered saline, or inoculated with either virulent or avirulent R. equi. NF-kappaB activation was increased in the nuclear extracts from foals inoculated with virulent R. equi at 14 days after inoculation when increased cytokine expression was also observed. Southwestern histochemistry revealed activated NF-kappaB in multinucleated giant cells that often contained bacteria. These results indicate that the cytokine response to R. equi is at least partially mediated by NF-kappaB activation.
先前的研究表明，与接种无毒质粒治愈衍生物的马驹相比，接种强毒马红球菌的马驹肺部白细胞介素-1β、白细胞介素-12 p40、干扰素-γ和肿瘤坏死因子-α mRNA水平显著更高。本研究的目的是确定细胞因子表达的增加是否与核因子-κB（NF-κB）肺部激活增加有关。对接种磷酸盐缓冲盐水、或接种强毒或无毒马红球菌的马驹提取的肺核蛋白进行电泳迁移率变动分析。接种强毒马红球菌的马驹在接种后14天，其核提取物中的NF-κB激活增加，此时也观察到细胞因子表达增加。蛋白质-DNA印迹组织化学显示，在通常含有细菌的多核巨细胞中存在激活的NF-κB。这些结果表明，对马红球菌的细胞因子反应至少部分由NF-κB激活介导。
