Kuwabara Mikio, Takuma Taishin, Scott Paul G, Dodd Carole M, Mizoguchi Itaru
Department of Orthodontics, School of Dentistry, Health Sciences University of Hokkaido, 1757 Kanazawa, Ishikari-Tobetsu, Hokkaido 061-0293, Japan.
Arch Oral Biol. 2002 Jun;47(6):473-80. doi: 10.1016/s0003-9969(02)00021-3.
To analyze the growth-related changes in extracellular matrix components, biochemical/immunohistochemical techniques were used to examine the protein expression and localization of two small leucine-rich proteoglycans, biglycan and decorin, in the temporomandibular joint discs of growing rats. Western blotting showed that the protein expression of decorin increased with age, but that of biglycan gradually decreased. An immunohistochemical study showed that staining for decorin was weak and homogeneously distributed in the discs from birth to 2 weeks. Regional differences in staining for decorin became prominent at 4, 8 and 16 weeks; decorin was more abundant in the peripheral area (the periphery of the band and the attachment) than in the central area (the intermediate zone and central area of the posterior band). In contrast, staining for biglycan was evenly distributed throughout the disc until 4 weeks, and after that became rather intense in the anterior and posterior bands. These results demonstrate that there are growth-related changes and regional differences in the expression of biglycan and decorin in the temporomandibular joint discs of growing rats, which probably reflect changes in the biomechanical environment caused by the development of orofacial functions.
为分析细胞外基质成分与生长相关的变化，采用生化/免疫组织化学技术检测了生长中大鼠颞下颌关节盘内两种富含亮氨酸的小分子蛋白聚糖（双糖链蛋白聚糖和核心蛋白聚糖）的蛋白表达及定位。蛋白质印迹法显示，核心蛋白聚糖的蛋白表达随年龄增长而增加，而双糖链蛋白聚糖的蛋白表达则逐渐降低。免疫组织化学研究表明，从出生到2周，核心蛋白聚糖在关节盘内的染色较弱且分布均匀。在4周、8周和16周时，核心蛋白聚糖染色的区域差异变得明显；核心蛋白聚糖在周边区域（带的周边和附着处）比在中央区域（后带的中间区和中央区）更为丰富。相比之下，双糖链蛋白聚糖的染色在4周前在整个关节盘内均匀分布，之后在前带和后带变得相当强烈。这些结果表明，生长中大鼠颞下颌关节盘内双糖链蛋白聚糖和核心蛋白聚糖的表达存在与生长相关的变化和区域差异，这可能反映了口面部功能发育引起的生物力学环境变化。
