Scott P G, Dodd C M, Tredget E E, Ghahary A, Rahemtulla F
Department of Oral Biology, University of Alberta, Edmonton, Canada.
Histopathology. 1995 May;26(5):423-31. doi: 10.1111/j.1365-2559.1995.tb00249.x.
The distributions of the small proteoglycans, decorin and biglycan and the large proteoglycan, versican, in normal skin and post-burn hypertrophic and mature scars, were compared using monoclonal and polyclonal antibodies to the core proteins. Biglycan and versican were virtually undetectable in normal dermis but readily seen in hypertrophic scars. Staining for decorin was strong throughout the dermis in normal skin but restricted to the deep dermis and a narrow zone under the epidermis in hypertrophic scar--areas which did not stain for versican. Decorin was absent or reduced in the nodules in these specimens. In mature post-burn scars, staining for all three proteoglycans demonstrated an intensity that was intermediate between that in normal dermis and that in the nodules of the hypertrophic scars. Transforming growth factor-beta was present in the nodules of hypertrophic scars but the deep dermis of these specimens stained most intensely for this cytokine which was also found in the dermis of mature scars but was not detectable in normal dermis. The apparent co-distribution of decorin and transforming growth factor-beta suggests that this proteoglycan may play an active role in the resolution of the scars. Changes in proteoglycan type and distribution could possibly account, at least in part, for the derangement of collagen and the altered physical properties of hypertrophic scar tissue.
使用针对核心蛋白的单克隆和多克隆抗体，比较了小蛋白聚糖、饰胶蛋白聚糖和双糖链蛋白聚糖以及大蛋白聚糖、多功能蛋白聚糖在正常皮肤、烧伤后增生性瘢痕和成熟瘢痕中的分布情况。在正常真皮中几乎检测不到双糖链蛋白聚糖和多功能蛋白聚糖，但在增生性瘢痕中很容易看到。在正常皮肤中，整个真皮中饰胶蛋白聚糖染色强烈，但在增生性瘢痕中仅限于真皮深层和表皮下的一个狭窄区域，而这些区域对多功能蛋白聚糖不染色。在这些标本的结节中，饰胶蛋白聚糖缺失或减少。在烧伤后成熟瘢痕中，所有三种蛋白聚糖的染色强度介于正常真皮和增生性瘢痕结节之间。转化生长因子-β存在于增生性瘢痕的结节中，但这些标本的真皮深层对这种细胞因子染色最强烈，在成熟瘢痕的真皮中也发现了这种细胞因子，但在正常真皮中检测不到。饰胶蛋白聚糖和转化生长因子-β的明显共同分布表明，这种蛋白聚糖可能在瘢痕的消退中发挥积极作用。蛋白聚糖类型和分布的变化可能至少部分地解释了增生性瘢痕组织中胶原蛋白的紊乱和物理性质的改变。
