Soupene Eric, King Natalie, Lee Haidy, Kustu Sydney
Department of Plant and Microbial Biology, University of California, Berkeley, California 94720-3102, USA.
J Bacteriol. 2002 Aug;184(15):4304-7. doi: 10.1128/JB.184.15.4304-4307.2002.
Transcription of an aqpZ-lac fusion in a single copy on the Escherichia coli chromosome increased as cells entered the stationary growth phase. This was true in a variety of media, and increased transcription in enriched medium required the RpoS sigma factor. Expression of the aqpZ-lac fusion was not affected by up- or downshifts in osmolality. Disruption of aqpZ had no detectable adverse effects.
在大肠杆菌染色体上以单拷贝形式存在的水通道蛋白Z(aqpZ)-乳糖操纵子融合基因的转录,随着细胞进入稳定生长期而增加。在多种培养基中均是如此,且在富集培养基中转录增加需要RpoS σ因子。aqpZ-乳糖操纵子融合基因的表达不受渗透压升高或降低的影响。aqpZ的缺失未产生可检测到的不利影响。