Schantz B
Acta Chir Scand. 1975;141(7):575-81.
Two autologous and 4 allogeneic conjunctival grafts have been transplanted to the corneal-scleral region in 6 dogs. The animals with the autografts and 2 of the allografts were untreated, while the animals with the other 2 allografts were treated with azathioprine and prednisolone. The grafts were followed with daily sequential, still photographic recording of the spreading of the fluorescence over the graft surfaces after fluorescein sodium had been given intravenously. A motordriven camera, with automatically timed exposures at very short intervals down to 1 sec, was used in combination with a fast recycling flash unit. The behaviour of the circulation seemed to be the same in auto- and in allografts during the first 8 days of healing. One day before showing signs of rejection at oridinary inspection, the two untreated allografts, on photographic analysis of the spreading of fluorescence, showed minute areas of non-fluorescence in an early stage of the procedure. The same type of local impairment of the circulation, manifested by slower uptake of dye in limited areas, was noted in the treated allografts, appearing 2-3 days before severe signs of rejection were noted at orinary inspection. These areas, however, gradually filled with dye during the further course of the test, to such a degree that the irregularities in the spreading of the fluorescence might easily have been overlooked in an oridinary fluorescein test. The disturbance of the circulation gradually increased during the course of the rejection and, at the stage when changes were observed at ordinary inspection, the circulation was severely impaired, as indicated by a complete non-appearance of fluorescence in certain areas, or in the whole graft. It is suggested that the modification of the classic fluorescein test described here, in detecting the early circulatory irregularities, indicates more precisely the start of the rejection process than do the criteria of allograft rejection used so far, and that it is, therefore, a sensitive means of evaluating the effects of various immunosuppressive procedures.
已将2个自体结膜移植物和4个异体结膜移植物移植到6只犬的角膜巩膜区域。接受自体移植物和2个异体移植物的动物未接受治疗，而接受另外2个异体移植物的动物用硫唑嘌呤和泼尼松龙治疗。静脉注射荧光素钠后，每天对移植物表面荧光扩散情况进行连续静态摄影记录，以此对移植物进行跟踪观察。使用一台电动相机，结合快速循环闪光灯单元，相机能以短至1秒的极短间隔自动定时曝光。在愈合的前8天，自体移植物和异体移植物中循环的表现似乎相同。在常规检查出现排斥迹象的前一天，对荧光扩散进行摄影分析时，两个未治疗的异体移植物在该过程的早期显示出微小的无荧光区域。在治疗的异体移植物中也观察到相同类型的局部循环障碍，表现为有限区域内染料摄取较慢，在常规检查发现严重排斥迹象前2 - 3天出现。然而，在测试的后续过程中，这些区域逐渐被染料填满，以至于在常规荧光素测试中，荧光扩散的不规则性很容易被忽略。在排斥过程中，循环障碍逐渐加剧，在常规检查观察到变化的阶段，循环严重受损，表现为某些区域或整个移植物完全无荧光。有人认为，这里描述的经典荧光素测试的改进方法，在检测早期循环不规则方面，比迄今使用的异体移植排斥标准更精确地表明了排斥过程的开始，因此，它是评估各种免疫抑制程序效果的一种敏感方法。
