Masadome Takashi, Asano Yasukazu, Imato Toshihiko, Ohkubo Satoshi, Tobita Tatsuya, Tabei Hisao, Iwasaki Yuzuru, Niwa Osamu, Fushinuki Yoshito
Department of Chemical Science and Engineering, Ariake National College of Technology, Omuta, Fukuoka 836-8585, Japan.
Anal Bioanal Chem. 2002 Jul;373(4-5):222-6. doi: 10.1007/s00216-002-1329-4. Epub 2002 May 24.
In order to simplify the procedure for assembling a surface-plasmon resonance (SPR) sensor, a refractive index matching polymer film was prepared as an alternative to the conventionally used matching oil. The refractive index matching polymer film, the refractive index of which was nearly equal to the prism and sensor chip material (a cover glass) of the SPR sensor, was prepared by casting a tetrahydrofuran solution of poly (vinyl chloride) (PVC) containing equal weights of dioctyl phthalate and tricresyl phosphate. The refractive index matching polymer film was found to have a refractive index of 1.516, which is identical to that of the prism and the cover glass used for the present SPR sensor. The utility of the matching polymer film for the SPR sensor was confirmed by the detection of anti-human albumin, based on an antigen-antibody reaction.
为了简化表面等离子体共振（SPR）传感器的组装过程，制备了一种折射率匹配聚合物膜，以替代传统使用的匹配油。通过浇铸含有等重量邻苯二甲酸二辛酯和磷酸三甲苯酯的聚氯乙烯（PVC）四氢呋喃溶液，制备了折射率与SPR传感器的棱镜和传感器芯片材料（盖玻片）几乎相等的折射率匹配聚合物膜。发现该折射率匹配聚合物膜的折射率为1.516，与用于本SPR传感器的棱镜和盖玻片的折射率相同。基于抗原-抗体反应对抗人白蛋白的检测证实了该匹配聚合物膜在SPR传感器中的实用性。
