Durán Merás I, Espinosa Mansilla A, Salinas López F, Rodríguez Gómez M J
Department of Analytical Chemistry, Faculty of Sciences, University of Extremadura, 06071 Badajoz, Spain.
Anal Bioanal Chem. 2002 Jul;373(4-5):251-8. doi: 10.1007/s00216-002-1348-1. Epub 2002 Jun 13.
Binary mixtures of methotrexate (MTX) and leucovorin (LV) have been resolved by application of first-derivative spectrophotometry and partial least squares calibration (PLS-1). By measuring the first-derivative signals of MTX and LV at 354 and 300 nm, respectively, simultaneous determination was possible. The mean recoveries for urine samples were 91 and 96% for MTX and LV, respectively. Partial least squares (PLS-1) multivariate calibration has been applied to the determination of these compounds in serum and in urine without pretreatment of the samples. The absorption spectra of serum or urine samples spiked with methotrexate and/or leucovorin, were used to optimize the calibration matrixes by the PLS-1 method. The sensitivity and selectivity of the proposed procedures were calculated. Mean recoveries were 101 and 97% for MTX and LV, respectively, for serum samples, and 101 and 98% for MTX and LV, respectively, for urine samples.
通过应用一阶导数分光光度法和偏最小二乘校准（PLS-1），已将甲氨蝶呤（MTX）和亚叶酸（LV）的二元混合物分离。通过分别测量MTX和LV在354和300 nm处的一阶导数信号，可以同时进行测定。尿液样品中MTX和LV的平均回收率分别为91%和96%。偏最小二乘（PLS-1）多元校准已应用于血清和尿液中这些化合物的测定，无需对样品进行预处理。用添加了甲氨蝶呤和/或亚叶酸的血清或尿液样品的吸收光谱，通过PLS-1方法优化校准矩阵。计算了所提出方法的灵敏度和选择性。血清样品中MTX和LV的平均回收率分别为101%和97%，尿液样品中MTX和LV的平均回收率分别为101%和98%。
