尿皮质素mRNA和肽在人前列腺及前列腺腺癌中的表达。
Expression of urocortin mRNA and peptide in the human prostate and in prostatic adenocarcinoma.
作者信息
Arcuri Felice, Cintorino Marcella, Florio Pasquale, Floccari Federica, Pergola Loreta, Romagnoli Roberta, Petraglia Felice, Tosi Piero, Teresa Del Vecchio Maria
机构信息
Institute of Pathological Anatomy and Histology, University of Siena, Siena, Italy.
出版信息
Prostate. 2002 Aug 1;52(3):167-72. doi: 10.1002/pros.10094.
BACKGROUND
Urocortin (UCN) is a recently described neuropeptide member of the CRF family, responsible for the secretion of the proopiomelanocortin-derived peptides from the pituitary gland. Although previous results have demonstrated the synthesis of several neuroendocrine factors in the prostate, no studies have been carried out on the expression of UCN in the human gland.
METHODS
UCN expression was evaluated in benign prostatic hyperplasia and prostatic tumor tissues by RT-PCR and immunohistochemistry.
RESULTS
UCN mRNA and peptide were demonstrated in all specimens tested. In nonneoplastic tissues, UCN was localized in the secretory luminal epithelial and basal layer cells, in the smooth muscle component of the stroma, and in lymphoid infiltrates. An intense immunostaining was evident in prostate adenocarcinoma cells.
CONCLUSIONS
The results of the present study demonstrate for the first time UCN expression in the human prostate and in prostate cancer, and suggest a potential involvement of UCN in prostate physiopathology.
背景
尿皮质素（UCN）是最近被描述的促肾上腺皮质激素释放因子（CRF）家族的神经肽成员，负责垂体中阿片黑素皮质素原衍生肽的分泌。尽管先前的研究结果已证明前列腺中可合成多种神经内分泌因子，但尚未对人前列腺中UCN的表达进行研究。
方法
采用逆转录聚合酶链反应（RT-PCR）和免疫组织化学方法评估良性前列腺增生和前列腺肿瘤组织中UCN的表达。
结果
在所有检测标本中均证实有UCN mRNA和肽存在。在非肿瘤组织中，UCN定位于分泌性管腔上皮和基底层细胞、基质的平滑肌成分以及淋巴浸润中。在前列腺腺癌细胞中可见强烈的免疫染色。
结论
本研究结果首次证明了UCN在人前列腺及前列腺癌中的表达，并提示UCN可能参与前列腺的生理病理过程。
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