Logan Malcolm, Martin James F, Nagy Andras, Lobe Corrinne, Olson Eric N, Tabin Clifford J
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA.
Genesis. 2002 Jun;33(2):77-80. doi: 10.1002/gene.10092.
We have used a Prx1 limb enhancer to drive expression of Cre Recombinase in transgenic mice. This regulatory element leads to Cre expression throughout the early limb bud mesenchyme and in a subset of craniofacial mesenchyme. Crossing a murine line carrying this transgene to a reporter mouse harboring a floxed Cre-reporter cassette revealed that recombinase activity is first observed in the earliest limb bud at 9.5 dpc. By early to mid bud stages at 10.5 dpc recombination is essentially complete in all mesenchymal cells in the limb. Expression of the Cre recombinase was never detected in the limb bud ectoderm. The use of Prx1-Cre mice should facilitate analysis of gene function in the developing limb.
我们已使用Prx1肢体增强子在转基因小鼠中驱动Cre重组酶的表达。这种调控元件导致Cre在整个早期肢体芽间充质以及颅面间充质的一个子集中表达。将携带该转基因的小鼠品系与携带loxP侧翼的Cre报告基因盒的报告小鼠杂交，结果显示在胚胎第9.5天最早的肢体芽中首次观察到重组酶活性。到胚胎第10.5天的芽早期至中期，肢体中所有间充质细胞的重组基本完成。在肢体芽外胚层中从未检测到Cre重组酶的表达。使用Prx1-Cre小鼠应有助于分析发育中肢体的基因功能。
