Sa Geng, Mi Ma, He-Chun Ye, Guo-Feng Li
Research Center of Plant Molecular and Developmental Biology, Institute of Botany, Chinese Academy of Sciences, Beijing, PR China.
Transgenic Res. 2002 Jun;11(3):269-78. doi: 10.1023/a:1015692127101.
Isopentenyl transferase (ipt) gene from Agrobacterium tumefaciens T-DNA was placed under the control of a TA29 promoter which expresses specifically in anther. The chimeric TA29-ipt gene was transferred to tobacco plants. During flowering, mRNA of the ipt gene in the anthers of the transgenic plants accumulated and the level of iPA + iPs increased 3-4-fold in the leaves, petals, pistils, and stamens compared with those in the wild type plants. This cytokinin increase affected various aspects in development indicating that the alterations of endogenous cytokinin level by using anther-specific expression of the TA29-ipt gene affected morphology, floral organ systems and reproductivity of the transgenic plants.
来自根癌农杆菌T-DNA的异戊烯基转移酶（ipt）基因置于花药中特异性表达的TA29启动子的控制之下。将嵌合的TA29-ipt基因导入烟草植株。在开花期间，转基因植株花药中ipt基因的mRNA积累，并且与野生型植株相比，叶片、花瓣、雌蕊和雄蕊中iPA + iPs的水平增加了3至4倍。这种细胞分裂素的增加影响了发育的各个方面，表明通过花药特异性表达TA29-ipt基因改变内源性细胞分裂素水平会影响转基因植株的形态、花器官系统和繁殖力。
