Sanno Naoko, Osamura Robert Y.
MD, PhD.
Endocr Pathol. 1998 Autumn;9(3):195-199. doi: 10.1007/BF02739958.
The catalyze reporter deposition (CARD) method is a new technique to enhance signal detection in immunostaining, immunoelectron microscopy, and in situ hybridization. This technique, which uses a biotinylated tyramine amplification reagent, has been applied to endocrine cells and tumors, and has shown a marked increase in sensitivity in detecting small amounts of protein antigens in endocrine cells and tumors. Although some technical problems remain before widespread application of this method can be used the potential for more in depth understanding of normal and neoplastic endocrine tissue by CARD analysis is largely unexplored at this time.
催化报告分子沉积（CARD）方法是一种用于增强免疫染色、免疫电子显微镜和原位杂交中信号检测的新技术。该技术使用生物素化酪胺扩增试剂，已应用于内分泌细胞和肿瘤，并且在检测内分泌细胞和肿瘤中的少量蛋白质抗原时显示出灵敏度显著提高。尽管在该方法能够广泛应用之前仍存在一些技术问题，但目前通过CARD分析更深入了解正常和肿瘤性内分泌组织的潜力在很大程度上尚未得到探索。
