Adrio Fátima, Anadón Ramón, Rodríguez-Moldes Isabel
Department of Fundamental Biology, Faculty of Biology, University of Santiago de Compostela, 15706 Santiago de Compostela, Spain.
J Comp Neurol. 2002 Jul 1;448(3):280-97. doi: 10.1002/cne.10256.
To obtain a better understanding of the evolution of the brain catecholaminergic systems of fishes, we have examined the distribution of catecholamine-synthesizing enzymes in two species of sturgeon (Acipenser baeri and Huso huso) using antibodies against tyrosine hydroxylase (TH) and dopamine-beta -hydroxylase (DBH; only analyzed in Acipenser). Both sturgeons showed TH-immunoreactive (THir) neurons widely distributed in most regions of the brain, the highest number of THir cells being located in the forebrain (olfactory bulb, preoptic area, and posterior tuberculum). THir cells were also seen in other forebrain areas (retrobulbar area, dorsal and ventral telencephalic areas, hypothalamus, ventral thalamus, pretectal area) and in the brainstem (locus coeruleus, viscerosensory area, caudal reticular formation, and area postrema). Immunoreactive fibers and varicosities showed a wide distribution, being particularly abundant in the diencephalon and mesencephalon. DBH-immunoreactive (DBHir) cells were observed in the anterior tuberal nucleus, where these cells were TH-negative, and in the locus coeruleus and the caudal rhombencephalon (vagal reticular formation), where the DBHir cells were also THir. DBHir fibers were scarce in the telencephalon and very abundant in the diencephalon, mesencephalon, and rhombencephalon. The comparative analysis of the catecholaminergic systems of chondrosteans and those observed in other groups of fishes and tetrapods indicate a similar organization of many nuclei, as well as characteristics that are probably primitive, such as the presence of a large number of forebrain catecholaminergic groups.
为了更好地了解鱼类脑内儿茶酚胺能系统的进化，我们使用抗酪氨酸羟化酶（TH）和多巴胺-β-羟化酶（DBH，仅在西伯利亚鲟中分析）的抗体，研究了两种鲟鱼（史氏鲟和欧洲鳇）中儿茶酚胺合成酶的分布。两种鲟鱼均显示TH免疫反应性（THir）神经元广泛分布于脑的大多数区域，THir细胞数量最多的位于前脑（嗅球、视前区和后结节）。在其他前脑区域（球后区、背侧和腹侧端脑区、下丘脑、腹侧丘脑、顶盖前区）和脑干（蓝斑、内脏感觉区、尾侧网状结构和最后区）也可见到THir细胞。免疫反应性纤维和膨体分布广泛，在间脑和中脑尤为丰富。在结节前核观察到DBH免疫反应性（DBHir）细胞，这些细胞TH呈阴性，在蓝斑和尾侧后脑（迷走网状结构）也观察到DBHir细胞，这些细胞也是THir。DBHir纤维在端脑稀少，在间脑、中脑和后脑非常丰富。对软骨硬鳞鱼类与其他鱼类和四足动物类群中儿茶酚胺能系统的比较分析表明，许多核团的组织方式相似，同时也具有一些可能是原始的特征，例如存在大量前脑儿茶酚胺能基团。
