Roca Anna, Abacassamo Fatima, Loscertales Mari-Paz, Quintó Llorenç, Gómez-Olivé Xavier, Fenwick Fiona, Saiz Juan Carlos, Toms Geoffrey, Alonso Pedro L
Unitat d'Epidemiologia i Bioestadística-UASP, Hospital Clínic, Universitat de Barcelona, Spain.
J Med Virol. 2002 Aug;67(4):616-23. doi: 10.1002/jmv.10148.
A case control study was carried out in Manhiça (Mozambique). Serum samples were collected from infants < 1 year of age in hospital to assess the effect of serum antibodies on the incidence of respiratory syncytial virus (RSV) infection. Sera were collected from a total of 31 cases of RSV infection and paired uninfected controls matched for age and sex. Anti-RSV antibodies were assessed by a membrane fluorescent antibody test (MFAT) for immunoglobulin G (IgG) antibodies and by a neutralizing antibody test. IgG RSV antibodies were of higher prevalence and at higher levels in the control group when compared to the infected case group (P < 0.001), indicating an important role for IgG antibodies in protection. To assess infection before recruitment, IgA RSV antibodies were also measured by MFAT. IgA RSV antibody prevalence was very low in patients and controls (0/31 and 4/31 respectively), suggesting that most of the detected IgG RSV antibody in both groups was of maternal origin. Re-analysis of data from the subset of 27 matched, IgA RSV antibody negative infant pairs mirrored the full analysis indicating that maternal antibody has an important role in RSV protection. Similar results were obtained when neutralizing antibodies were measured and when the measurement was done against subgroup A virus strain A2, subgroup B virus strain 8/60 and a contemporary subgroup A isolate, Moz00. No significant differences in the reactivity of maternal antibodies with the three virus strains were observed. The data described below represent the first analysis of the role of maternal antibodies in reducing the risk of pediatric infection in developing countries. The results reinforce the concept of maternal vaccination for the control of RSV in very young children in whom the risk and severity of infection are the highest.
在莫桑比克的曼希卡开展了一项病例对照研究。从住院的1岁以下婴儿中采集血清样本，以评估血清抗体对呼吸道合胞病毒（RSV）感染发生率的影响。共采集了31例RSV感染病例以及年龄和性别匹配的未感染对照的血清。通过膜荧光抗体试验（MFAT）检测免疫球蛋白G（IgG）抗体，并通过中和抗体试验评估抗RSV抗体。与感染病例组相比，对照组中IgG RSV抗体的患病率更高且水平更高（P < 0.001），表明IgG抗体在保护中起重要作用。为了评估入组前的感染情况，还通过MFAT检测了IgA RSV抗体。患者和对照组中IgA RSV抗体的患病率都非常低（分别为0/31和4/31），这表明两组中检测到的大多数IgG RSV抗体都来自母体。对27对匹配的、IgA RSV抗体阴性婴儿亚组的数据重新分析反映了全面分析的结果，表明母体抗体在RSV保护中起重要作用。当检测中和抗体以及针对A亚组病毒株A2、B亚组病毒株8/60和当代A亚组分离株Moz00进行检测时，也获得了类似结果。未观察到母体抗体与这三种病毒株反应性的显著差异。以下所述数据代表了对发展中国家母体抗体在降低儿童感染风险中作用的首次分析。结果强化了母体接种疫苗以控制RSV感染风险和严重程度最高的幼儿RSV感染的概念。
