Chen Wen, Palmer Robert J, Kuramitsu Howard K
Department of Oral Biology, State University of New York, Buffalo 14214, USA.
Infect Immun. 2002 Aug;70(8):4708-15. doi: 10.1128/IAI.70.8.4708-4715.2002.
In order to assess the role of polyphosphate kinase (PPK) in the physiology of Porphyromonas gingivalis, a ppk gene mutant, CW120, was constructed and characterized. P. gingivalis was demonstrated to synthesize short-chain polyphosphate (polyP) but not long-chain polyP. CW120 failed to survive in the stationary phase as well as the parental cell did, and it was attenuated in biofilm formation on polyvinylchloride and glass surfaces. Furthermore, the complementation by insertion of an intact copy of the ppk gene into the mutant CW120 restored its biofilm formation and stationary-phase survival. These results suggest that PPK may be important for incorporation of these organisms into subgingival plaque in the human oral cavity.
为了评估多聚磷酸激酶(PPK)在牙龈卟啉单胞菌生理学中的作用,构建并鉴定了一个ppk基因突变体CW120。牙龈卟啉单胞菌被证明能合成短链多聚磷酸(polyP),但不能合成长链多聚磷酸。CW120在稳定期无法像亲代细胞那样存活,并且在聚氯乙烯和玻璃表面形成生物膜的能力减弱。此外,将完整的ppk基因拷贝插入突变体CW120中进行互补,恢复了其生物膜形成能力和稳定期存活率。这些结果表明,PPK对于这些微生物在人类口腔龈下菌斑中的定植可能很重要。