海马内微量注射毛果芸香碱诱发癫痫发作的行为学、形态学及脑电图评估
Behavioral, morphologic, and electroencephalographic evaluation of seizures induced by intrahippocampal microinjection of pilocarpine.
作者信息
Furtado Marcio de A, Braga Glaucia K, Oliveira José A C, Del Vecchio Flavio, Garcia-Cairasco Norberto
机构信息
Neurophysiology and Experimental Neuroethology Laboratory, Physiology Department, Ribeirão Preto School of Medicine, University of São Paulo, 14049-900 Ribeirão Preto, SP, Brazil.
出版信息
Epilepsia. 2002;43 Suppl 5:37-9. doi: 10.1046/j.1528-1157.43.s.5.41.x.
PURPOSE
We studied, by means of video-EEG and neo-Timm histochemistry, the behavioral, electrophysiologic, and structural characteristics of seizures induced by intrahippocampal microinjection of pilocarpine (HIP-PILO), a selective model of temporal lobe epilepsy (TLE).
METHODS
We investigated the behavioral and electrophysiologic (hippocampus and amygdala EEG) evaluation of status epilepticus (SE) induced by HIP-PILO and the consequent spontaneous recurrent seizures (SRSs). We evaluated hippocampal structural rearrangements after SE by means of neo-Timm staining.
RESULTS
HIP-PILO induced SE in 17 (71%) of 24 animals. Although three animals displayed spontaneous remission of SE (not used in analysis) before the established SE duration (90 min), none of those undergoing SE died. Of SE animals, 10 (71%) of 14 had SRSs. All animals with SE had clear-cut mossy fiber sprouting (MFS) in the inner molecular layer of the dentate gyrus and epileptiform activity in hippocampus and amygdala.
CONCLUSIONS
HIP-PILO rats displayed SE, SRS, MFS, and limbic epileptiform activity, without animal loss after SE. Thus, our data support this as a selective and efficient model of TLE.
目的
我们通过视频脑电图和新蒂姆组织化学方法，研究了海马内微量注射毛果芸香碱（HIP-PILO）诱发的癫痫发作的行为、电生理和结构特征，HIP-PILO是颞叶癫痫（TLE）的一种选择性模型。
方法
我们研究了HIP-PILO诱发的癫痫持续状态（SE）及其后续的自发性反复癫痫发作（SRSs）的行为和电生理（海马和杏仁核脑电图）评估。我们通过新蒂姆染色评估了SE后海马的结构重排。
结果
24只动物中有17只（71%）被HIP-PILO诱发了SE。虽然有3只动物在既定的SE持续时间（90分钟）之前出现了SE的自发缓解（未用于分析），但所有经历SE的动物均未死亡。在SE动物中，14只中有10只（71%）出现了SRSs。所有出现SE的动物在齿状回内分子层均有明显的苔藓纤维发芽（MFS），海马和杏仁核有癫痫样活动。
结论
HIP-PILO大鼠表现出SE、SRS、MFS和边缘性癫痫样活动，SE后无动物死亡。因此，我们的数据支持这是一种TLE的选择性和有效模型。
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