Lemos T, Cavalheiro E A
Neurologia Experimental, Escola Paulista de Medicina, São Paulo, Brazil.
Exp Brain Res. 1995;102(3):423-8. doi: 10.1007/BF00230647.
Status epilepticus (SE) has been related to subsequent development of epilepsy. The present work was aimed at elucidating the relationship between the duration of pilocarpine- (PILO)-induced SE and the subsequent development of epilepsy in rats. The latency for the appearance of the first spontaneous seizure, the frequency of spontaneous seizures, the cell density in the hippocampal formation and the density of supragranular neo-Timm staining were monitored. At 30 min, 1, 2 and 6 h after the beginning of SE, animals were treated with diazepam plus pentobarbital. In non-treated rats, SE remitted spontaneously. Animals exhibiting 30 min of PILO-induced SE did not develop spontaneous seizures. Hippocampal cell counts and the density of neo-Timm staining in these animals were similar to those observed in control rats. In the other groups longer SE durations were related to: shorter latency for the appearance of the first spontaneous seizure, increased number of the spontaneous recurrent seizures, severe cell loss in the hippocampal formation, or increased supragranular neo-Timm staining. These data suggest that more than 30 min of SE is required to produce hippocampal damage with subsequent synaptic reorganization of the mossy fibre pathway that could account for SRSs observed in the PILO model of epilepsy.
癫痫持续状态（SE）与随后癫痫的发生有关。本研究旨在阐明毛果芸香碱（PILO）诱导的SE持续时间与大鼠随后癫痫发生之间的关系。监测首次自发性癫痫发作出现的潜伏期、自发性癫痫发作的频率、海马结构中的细胞密度以及颗粒上层新Timm染色的密度。在SE开始后的30分钟、1小时、2小时和6小时，给动物注射地西泮加戊巴比妥。在未治疗的大鼠中，SE自发缓解。表现出30分钟PILO诱导的SE的动物未出现自发性癫痫发作。这些动物的海马细胞计数和新Timm染色密度与对照大鼠相似。在其他组中，较长的SE持续时间与以下情况有关：首次自发性癫痫发作出现的潜伏期缩短、自发性复发性癫痫发作次数增加、海马结构中严重的细胞丢失或颗粒上层新Timm染色增加。这些数据表明，需要超过30分钟的SE才能造成海马损伤，并随后导致苔藓纤维通路的突触重组，这可能解释了在PILO癫痫模型中观察到的自发性复发性癫痫发作。
