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实验性急性胰腺炎期间大鼠外分泌胰腺中分泌应激蛋白(PSP/reg、PAP I、PAP II和PAP III)的协调调节

Coordinate regulation of secretory stress proteins (PSP/reg, PAP I, PAP II, and PAP III) in the rat exocrine pancreas during experimental acute pancreatitis.

作者信息

Graf R, Schiesser M, Lüssi A, Went Ph, Scheele G A, Bimmler D

机构信息

Pancreatitis Research Laboratory, Department of Visceral and Transplant Surgery, University Hospital, 8091 Zürich, Switzerland.

出版信息

J Surg Res. 2002 Jun 15;105(2):136-44. doi: 10.1006/jsre.2002.6387.

Abstract

BACKGROUND

Pancreatic stone protein (PSP/reg) is a constitutively secreted protein in pancreatic juice. Pancreatitis-associated protein (PAP) belongs to the same family of proteins. PAP is highly increased during acute pancreatitis, while no exact data exist regarding PSP/reg protein synthesis and secretion. Recently, an attempt to determine PSP/reg and PAP levels in sera of rats with acute pancreatitis showed a significant increase in PAP but failed to demonstrate changes in PSP/reg. Others reported that surgical manipulation of the pancreas, including sham controls, affected mRNA levels of PSP/reg. Neither report determined protein levels of PSP/reg.

METHODS

Rats were treated intraperitoneally with a supramaximal dose of caerulein to induce pancreatitis, a physiological dose of caerulein, or a saline injection. Pancreata were analyzed for PAP and PSP/reg using ELISAs. RNA was extracted for Northern blot analysis of PAP I, II, and III and PSP/reg mRNA.

RESULTS

Experimental induction of acute pancreatitis caused a coordinate increase in both PSP/reg and PAP. PAP showed an acute response and returned to low levels within 48 h while PSP/reg exhibited a more sustained response. Intraperitoneal application of a physiological dose of caerulein and even a saline injection caused an increase in PSP/reg.

CONCLUSION

PSP/reg and PAP levels are increased through similar mechanisms by physiological and supramaximal doses of caerulein. However, PSP/reg regulation appears to sustain high levels while PAP levels are more transient. Since the regulation of this protein family is affected even under mild stress, we define them as secretory stress proteins.

摘要

背景

胰石蛋白(PSP/reg)是胰液中一种组成性分泌的蛋白质。胰腺炎相关蛋白(PAP)属于同一蛋白家族。PAP在急性胰腺炎期间显著升高,而关于PSP/reg蛋白合成和分泌尚无确切数据。最近,一项测定急性胰腺炎大鼠血清中PSP/reg和PAP水平的尝试显示PAP显著升高,但未能证明PSP/reg有变化。其他人报告称,胰腺的手术操作,包括假手术对照组,会影响PSP/reg的mRNA水平。两份报告均未测定PSP/reg的蛋白水平。

方法

给大鼠腹腔注射超最大剂量的蛙皮素以诱导胰腺炎、生理剂量的蛙皮素或注射生理盐水。使用酶联免疫吸附测定法(ELISA)分析胰腺中的PAP和PSP/reg。提取RNA用于对PAP I、II和III以及PSP/reg mRNA进行Northern印迹分析。

结果

实验性诱导急性胰腺炎导致PSP/reg和PAP同时升高。PAP表现出急性反应,并在48小时内恢复到低水平,而PSP/reg表现出更持久的反应。腹腔注射生理剂量的蛙皮素甚至注射生理盐水都会导致PSP/reg升高。

结论

生理剂量和超最大剂量的蛙皮素通过相似机制使PSP/reg和PAP水平升高。然而,PSP/reg的调节似乎维持在高水平,而PAP水平更短暂。由于即使在轻度应激下该蛋白家族的调节也会受到影响,我们将它们定义为分泌性应激蛋白。

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