Yung K H, Northcote D H
Biochem J. 1975 Oct;151(1):141-4. doi: 10.1042/bj1510141.
Cell-wall enzymes were assayed by the difference between enzyme activities in the whole cell and the protoplast. Both peroxidase (85.2%) and acid phosphatase (21.9%) were located in the wall. However, malate dehydrogenase was found only in the protoplast. A study of the time-course of the release of peroxidase and malate dehydrogenase into the incubation medium from cells either treated with cellulase or untreated, also indicated that peroxidase and not malate dehydrogenase was located in the wall. Only two anodic isoenzymes of peroxidase were present in the cell wall. These were more negatively charged than those of horseradish peroxidase.
通过测定全细胞和原生质体中酶活性的差异来分析细胞壁酶。过氧化物酶(85.2%)和酸性磷酸酶(21.9%)都位于细胞壁中。然而,苹果酸脱氢酶仅存在于原生质体中。对用纤维素酶处理或未处理的细胞中过氧化物酶和苹果酸脱氢酶释放到培养液中的时间进程研究也表明,过氧化物酶而非苹果酸脱氢酶位于细胞壁中。细胞壁中仅存在两种过氧化物酶阳极同工酶。它们比辣根过氧化物酶的同工酶带更多负电荷。