Press Michael, Spaulding Betsy, Groshen Susan, Kaminsky David, Hagerty Margaret, Sherman Lori, Christensen Kurt, Edwards Dean P
Department of Pathology, University of Southern California School of Medicine, Los Angeles, CA 90033, USA.
Steroids. 2002 Aug;67(9):799-813. doi: 10.1016/s0039-128x(02)00039-9.
Monoclonal antibodies directed against human estrogen receptor (ER) and progesterone receptor (PR) have been used extensively for biochemical and immunohistochemical detection of receptors independent of hormone-binding assays. These antibodies have been valuable both for experimental work and for detection of receptors in clinical breast cancer specimens. The purpose of this study was to characterize the sensitivity and specificity of different antibodies for detection of PR by immunohistochemistry (IHC) of formalin-fixed paraffin breast carcinoma sections. The panel of twelve antibodies included two new ones (PgR636 and PgR1294) produced prospectively to be resistant to formalin fixation and paraffin embedding. Fifty-nine breast carcinomas, having known PR levels by biochemical ligand-binding assay, were used to prepare multitumor paraffin-embedded tissue blocks for characterization of the PR antibodies. Of all the antibodies tested, both PgR636 and PgR1294 stained the highest percentage of breast carcinomas known to be positive by the biochemical assay (95-98%) and they exhibited the highest concordance with the biochemical assay (88-90%). The PgR636 and PgR1294 antibodies, along with one other, PR 88, also gave the highest intensity of nuclear staining, while PgR636 and PgR1294 stained the highest mean percentage of tumor cell nuclei. Antigen retrieval was not necessary for PR immunostaining by PgR636 and PgR1294 in most tumors and other tissues examined, but did slightly increase the staining intensity. The majority of the other antibodies tested were highly dependent on antigen retrieval; only PR 88 and KD 68 antibodies approached the performance of PgR636 and PgR1294 without antigen retrieval. These results indicate that PgR636 and PgR1294 are optimal antibodies for IHC detection of PR in routine paraffin tissue blocks.
针对人雌激素受体（ER）和孕激素受体（PR）的单克隆抗体已广泛用于独立于激素结合测定的受体生化和免疫组织化学检测。这些抗体对于实验工作以及临床乳腺癌标本中受体的检测都很有价值。本研究的目的是通过福尔马林固定石蜡包埋的乳腺癌切片免疫组织化学（IHC）来表征不同抗体检测PR的敏感性和特异性。十二种抗体组成的抗体组包括两种新抗体（PgR636和PgR1294），这两种抗体是前瞻性生产的，对福尔马林固定和石蜡包埋具有抗性。59例通过生化配体结合测定已知PR水平的乳腺癌用于制备多肿瘤石蜡包埋组织块，以表征PR抗体。在所有测试的抗体中，PgR636和PgR1294对生化测定已知为阳性的乳腺癌染色百分比最高（95 - 98%），并且它们与生化测定的一致性最高（88 - 90%）。PgR636和PgR1294抗体以及另一种PR 88抗体也给出了最高强度的核染色，而PgR636和PgR1294对肿瘤细胞核的平均染色百分比最高。在大多数检测的肿瘤和其他组织中，PgR636和PgR1294进行PR免疫染色时不需要抗原修复，但确实会略微增加染色强度。大多数其他测试抗体高度依赖抗原修复；只有PR 88和KD 68抗体在不进行抗原修复的情况下接近PgR636和PgR1294的性能。这些结果表明，PgR636和PgR1294是常规石蜡组织块中PR免疫组织化学检测的最佳抗体。
