Ozzello L, DeRosa C, Habif D V, Greene G L
Arthur Purdy Stout Laboratory of Surgical Pathology, Columbia University, New York, New York.
Cancer. 1991 Jan 15;67(2):455-62. doi: 10.1002/1097-0142(19910115)67:2<455::aid-cncr2820670223>3.0.co;2-m.
Two monoclonal antibodies to progesterone receptor (PR), JZB39 and KD68, were used for the immunocytochemical visualization of PR in different kinds of breast cancer specimens including (1) cryostat sections of tumors frozen at -80 degrees C; (2) paraffin sections of tumors fixed in formalin or in Bouin's fixative for varying periods of time at room temperature or at 4 degrees C; and (3) imprints and cryostat sections prepared from the tissue used for frozen section diagnosis and stored at -80 degrees C after fixation in Zamboni's solution. Sections of conventionally frozen specimens as well as imprints and cryostat sections stored for varying periods of time were stained with the peroxidase-antiperoxidase technique, whereas the avidin-biotin technique was used for paraffin sections. In all types of specimens the PR immunostaining was localized to the nuclei of carcinoma cells and displayed considerable heterogeneity both in intensity and in distribution of positive cells. Close correspondence was found between the different immunohistochemical techniques as well as between immunostaining and steroid-binding assays. PR staining was more frequently positive in well-differentiated than in moderately or poorly differentiated carcinomas, whereas no meaningful correlation was found between PR staining and extent of the disease. Similar results were obtained with the immunostaining of estrogen receptor in the same material using monoclonal antibodies H222 and D75P3 gamma. Thus, by choosing the technique that best suits the type of specimen available, it is possible to obtain valid information on the receptor status of any breast carcinoma, regardless of its size and clinical presentation.
两种针对孕激素受体（PR）的单克隆抗体JZB39和KD68，用于对不同类型乳腺癌标本中的PR进行免疫细胞化学可视化，这些标本包括：（1）在-80℃冷冻的肿瘤低温切片；（2）在室温或4℃下用福尔马林或Bouin固定液固定不同时间的肿瘤石蜡切片；（3）取自用于冷冻切片诊断的组织并在Zamboni溶液中固定后储存在-80℃的印片和低温切片。常规冷冻标本的切片以及储存不同时间的印片和低温切片用过氧化物酶-抗过氧化物酶技术染色，而石蜡切片则使用抗生物素蛋白-生物素技术。在所有类型的标本中，PR免疫染色定位于癌细胞的细胞核，并且在阳性细胞的强度和分布上均表现出相当大的异质性。在不同的免疫组织化学技术之间以及免疫染色和类固醇结合测定之间发现了密切的对应关系。PR染色在高分化癌中比在中分化或低分化癌中更频繁地呈阳性，而PR染色与疾病范围之间未发现有意义的相关性。使用单克隆抗体H222和D75P3γ对相同材料中的雌激素受体进行免疫染色也获得了类似的结果。因此，通过选择最适合现有标本类型的技术，无论乳腺癌的大小和临床表现如何，都有可能获得关于其受体状态的有效信息。
