一种新型小鼠肝脏醛酮还原酶AKR7A5的特性分析

Characterisation of a novel mouse liver aldo-keto reductase AKR7A5.

作者信息

Hinshelwood Alison, McGarvie Gail, Ellis Elizabeth

机构信息

Department of Pharmaceutical Sciences, University of Strathclyde, 204 George Street, G1 1XW, Glasgow, UK.

出版信息

FEBS Lett. 2002 Jul 17;523(1-3):213-8. doi: 10.1016/s0014-5793(02)02982-4.

DOI:10.1016/s0014-5793(02)02982-4

PMID:12123834

Abstract

We have characterised a novel aldo-keto reductase (AKR7A5) from mouse liver that is 78% identical to rat aflatoxin dialdehyde reductase AKR7A1 and 89% identical to human succinic semialdehyde (SSA) reductase AKR7A2. AKR7A5 can reduce 2-carboxybenzaldehyde (2-CBA) and SSA as well as a range of aldehyde and diketone substrates. Western blots show that it is expressed in liver, kidney, testis and brain, and at lower levels in skeletal muscle, spleen heart and lung. The protein is not inducible in the liver by dietary ethoxyquin. Immunodepletion of AKR7A5 from liver extracts shows that it is one of the major liver 2-CBA reductases but that it is not the main SSA reductase in this tissue.

摘要

我们已经鉴定出一种来自小鼠肝脏的新型醛酮还原酶（AKR7A5），它与大鼠黄曲霉毒素二醛还原酶AKR7A1有78%的同源性，与人类琥珀酸半醛（SSA）还原酶AKR7A2有89%的同源性。AKR7A5能够还原2-羧基苯甲醛（2-CBA）和SSA以及一系列醛和二酮底物。蛋白质免疫印迹显示它在肝脏、肾脏、睾丸和大脑中表达，在骨骼肌、脾脏、心脏和肺中的表达水平较低。该蛋白在肝脏中不受膳食乙氧喹的诱导。从肝脏提取物中免疫去除AKR7A5表明，它是肝脏中主要的2-CBA还原酶之一，但不是该组织中主要的SSA还原酶。