Ernst C, Lematre J, Rinnert H, Dupont G, Grange J
C R Seances Acad Sci D. 1979 Dec 10;289(15):1145-8.
The polyene antibiotic "Amphotericin B" can interact with sterols, cholesterol or ergosterol in aqueous and hydroalcoholic media and a correlative striking spectral change appears between 300 and 420 nm in the CD and absorption spectra. Using these spectroscopic methods we have determined that the influence of temperature between 4 and 80 degrees C is very important. The higher the temperature the most rapid is the modification of the spectra. Thus, the "Amphotericin B"-sterol complex is more easily formed when heating. We have not found any reversibility at 80 degrees C.
多烯抗生素“两性霉素B”在水性和水醇性介质中可与甾醇、胆固醇或麦角固醇相互作用，并且在圆二色光谱（CD）和吸收光谱中，300至420纳米之间会出现显著的相关光谱变化。利用这些光谱方法，我们已确定4至80摄氏度之间的温度影响非常重要。温度越高，光谱变化越快。因此，加热时“两性霉素B”-甾醇复合物更容易形成。我们发现在80摄氏度时不存在任何可逆性。
