Cao Bo-Jin, Reith Maarten E A
Department of Biomedical and Therapeutic Sciences, University of Illinois College of Medicine, Peoria, IL 61656-1649, USA.
Eur J Pharmacol. 2002 Jul 12;448(1):27-30. doi: 10.1016/s0014-2999(02)01908-8.
2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (carboxy-PTIO) has been increasingly used as nitric oxide (NO) scavenger. Carboxy-PTIO reacts with NO to form nitric dioxide and 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl (carboxy-PTI). In rat C6 glioma cells expressing human dopamine transporter, carboxy-PTIO paradoxically potentiated the inhibition of [(3)H]dopamine uptake by two NO donors, diethylamine/NO and (Z)-1-[N-(3-ammoniopropyl)-N-(n-propyl)-amino]/NO. Further examinations revealed that carboxy-PTI concentration-dependently reduced dopamine uptake, indicating that the formation of carboxy-PTI may account for the failure of carboxy-PTIO to abolish NO elicited effects. These results suggest that caution should be taken in interpreting data obtained using carboxy-PTIO and probably other NO scavengers.
2-(4-羧基苯基)-4,4,5,5-四甲基咪唑啉-1-氧基-3-氧化物（羧基-PTIO）已越来越多地用作一氧化氮（NO）清除剂。羧基-PTIO与NO反应生成二氧化氮和2-(4-羧基苯基)-4,4,5,5-四甲基咪唑啉-1-氧基（羧基-PTI）。在表达人多巴胺转运体的大鼠C6胶质瘤细胞中，羧基-PTIO出人意料地增强了两种NO供体二乙胺/NO和(Z)-1-[N-(3-氨丙基)-N-(正丙基)-氨基]/NO对[(3)H]多巴胺摄取的抑制作用。进一步研究表明，羧基-PTI浓度依赖性地降低多巴胺摄取，这表明羧基-PTI的形成可能是羧基-PTIO未能消除NO引发效应的原因。这些结果表明，在解释使用羧基-PTIO以及可能其他NO清除剂获得的数据时应谨慎。
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