一氧化氮清除剂羧基-2-（4-羧基苯基）-4,4,5,5-四甲基咪唑啉-1-氧基-3-氧化物（carboxy-PTIO）对大鼠异氟烷最低肺泡有效浓度（MAC）和小脑一氧化氮合酶活性的影响。

Effects of a nitric oxide scavenger, carboxy-PTIO, on isoflurane MAC and cerebellar nitric oxide synthase activity in rats.

作者信息

Chen T Y, Chang C L, Tseng C C, Cheng J T

机构信息

Department of Anaesthesiology, College of Medicine, National Cheng Kung University, Tainan City, Taiwan, Republic of China.

出版信息

Br J Anaesth. 1999 Dec;83(6):948-50. doi: 10.1093/bja/83.6.948.

DOI:10.1093/bja/83.6.948

PMID:10700799

Abstract

Recent studies have indicated that nitric oxide may play a role in inhalation anaesthesia. Inhibition of nitric oxide synthase reduces the minimum alveolar concentration (MAC) for inhalation anaesthetics and decreases cerebellar nitric oxide synthase (NOS) activity in rats. In this study, we have explored further the role of nitric oxide in isoflurane anaesthesia by examining the effects of a nitric oxide scavenger, 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (carboxy-PTIO 0.075-0.6 mg kg-1) on MAC values and cerebellar NOS activity in rats. Bolus injection of carboxy-PTIO at doses greater than 0.15 mg kg-1 reduced the MAC value of isoflurane (mean 1.36 (SEM 0.07)% at 0.15 mg kg-1, 1.39 (0.14)% at 0.3 mg kg-1 and 1.31 (0.06)% at 0.6 mg kg-1 vs control value of 1.61 (0.19)%. Administration of carboxy-PTIO 0.125 and 0.15 mg kg-1 resulted in increased cerebellar NOS activity during isoflurane anaesthesia (P < 0.05). These findings suggest that the level of nitric oxide may set a baseline from which isoflurane then acts.

摘要

最近的研究表明，一氧化氮可能在吸入麻醉中发挥作用。抑制一氧化氮合酶可降低吸入麻醉药的最低肺泡浓度（MAC），并降低大鼠小脑一氧化氮合酶（NOS）的活性。在本研究中，我们通过检测一氧化氮清除剂2-（4-羧基苯基）-4,4,5,5-四甲基咪唑啉-1-氧基-3-氧化物（羧基-PTIO，0.075 - 0.6 mg kg-1）对大鼠MAC值和小脑NOS活性的影响，进一步探讨了一氧化氮在异氟烷麻醉中的作用。剂量大于0.15 mg kg-1的羧基-PTIO静脉推注可降低异氟烷的MAC值（0.15 mg kg-1时平均为1.36（标准误0.07）%，0.3 mg kg-1时为1.39（0.14）%，0.6 mg kg-1时为1.31（0.06）%，而对照值为1.61（0.19）%）。给予0.125和0.15 mg kg-1的羧基-PTIO可导致异氟烷麻醉期间小脑NOS活性增加（P < 0.05）。这些发现表明，一氧化氮水平可能设定了一个基线，异氟烷随后在此基础上发挥作用。