液相色谱 - 质谱法测定人血浆和尿液中的氟哌啶醇及其代谢物。
Liquid chromatographic-mass spectrometric determination of haloperidol and its metabolites in human plasma and urine.
作者信息
Arinobu Tetsuya, Hattori Hideki, Iwai Masae, Ishii Akira, Kumazawa Takeshi, Suzuki Osamu, Seno Hiroshi
机构信息
Department of Legal Medicine, Aichi Medical University School of Medicine, Nagakute-cho, Aichi 480-1195, Japan.
出版信息
J Chromatogr B Analyt Technol Biomed Life Sci. 2002 Aug 25;776(1):107-13. doi: 10.1016/s1570-0232(02)00175-7.
Haloperidol and its two metabolites, reduced haloperidol and 4-(4-chlorophenyl)-4-hydroxypiperidine (CPHP) in human plasma and urine were analyzed by HPLC-MS using a new polymer column (MSpak GF-310), which enabled direct injection of crude biological samples without pretreatment. Recoveries of haloperidol and reduced haloperidol spiked into plasma were 64.4-76.1% and 46.8-50.2%, respectively; those for urine were 87.3-99.4% and 94.2-98.5%, respectively; those of CPHP for both samples were not less than 92.7%. The regression equations for haloperidol, reduced haloperidol and CPHP showed good linearity in the ranges of 10-800, 15-800 and 400-800 ng/ml, respectively, for both plasma and urine. Their detection limits were 5, 10 and 300 ng/ml, respectively, for both samples. Thus, the present method was sensitive enough for detection and determination of high therapeutic and toxic levels for haloperidol and its metabolites present in biological samples.
采用新型聚合物柱（MSpak GF - 310），通过高效液相色谱 - 质谱联用（HPLC - MS）法分析人血浆和尿液中的氟哌啶醇及其两种代谢产物，即还原氟哌啶醇和4 - （4 - 氯苯基） - 4 - 羟基哌啶（CPHP）。该方法可直接进样未经预处理的生物粗样品。加标于血浆中的氟哌啶醇和还原氟哌啶醇的回收率分别为64.4% - 76.1%和46.8% - 50.2%；加标于尿液中的回收率分别为87.3% - 99.4%和94.2% - 98.5%；两种样品中CPHP的回收率均不低于92.7%。氟哌啶醇、还原氟哌啶醇和CPHP的回归方程在血浆和尿液中的线性范围分别为10 - 800、15 - 800和400 - 800 ng/ml。两种样品中它们的检测限分别为5、10和300 ng/ml。因此，本方法对于检测和测定生物样品中氟哌啶醇及其代谢产物的高治疗浓度和中毒浓度足够灵敏。
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