Segal A W, Levi A J
Clin Exp Immunol. 1975 Feb;19(2):309-18.
Nitroblue tetrazolium (NBT) is toxic to neutrophils; an effect which is greatly enhanced by endotoxin and latex particles. Cell damage, measured by the release of the cytoplasmic marker enzyme lactate dehydrogenase (LDH), was closely related to dye reduction. This suggests that, in this test, dye reduction occurs largely as a result of contact between intracellular reducing compounds and NBT following damage of the outer cell membrane. The expression of dye reduction as a function of LDH release should enhance the sensitivity of the quantitative NBT test by correcting for the observed intersubject variation in cell damage. The relationship between cell damage and dye reduction is a measure of the reducing capacity of the cell. This was normal in immature, bone marrow neutrophils, but diminished in neutrophils of patients with chronic granulomatous disease.
硝基蓝四氮唑（NBT）对中性粒细胞有毒性；内毒素和乳胶颗粒会大大增强这种毒性作用。通过细胞质标记酶乳酸脱氢酶（LDH）的释放来衡量的细胞损伤与染料还原密切相关。这表明，在该试验中，染料还原很大程度上是由于外细胞膜受损后细胞内还原化合物与NBT接触所致。将染料还原表达为LDH释放的函数，应通过校正观察到的受试者间细胞损伤差异来提高定量NBT试验的灵敏度。细胞损伤与染料还原之间的关系是细胞还原能力的一种度量。这在未成熟的骨髓中性粒细胞中是正常的，但在慢性肉芽肿病患者的中性粒细胞中则降低。
