Troum S, Dalton M L
Department of Surgery, Mercer University School of Medicine, Medical Center of Central Georgia, Macon, USA.
J South Orthop Assoc. 2001 Spring;10(1):37-43.
We investigated the use of a biodegradable porcine gelatin matrix (Gelfoam) as a carrier for marrow cells that induce osteogenesis at ectopic sites in rats. Bone marrow cells obtained from the long bones of 6-week-old Sprague-Dawley rats were dissociated enzymatically and the cells reconstituted in rat serum. Twenty million cells in 0.1 mL of serum were then adsorbed into 1-cm3 pieces of gelatin matrix substrate and implanted into ectopic sites in live Sprague-Dawley rats. The implants were retrieved and analyzed histologically for bone and cartilage formation 3, 4, 6, and 8 weeks after implantation. Woven bone was seen as early as 3 weeks and persisted through 8 weeks. No cartilage was observed. Osteoclasts first appeared at 3 weeks, peaking in number at 4 weeks. By 6 and 8 weeks, only small islets of substrate remained, surrounded by dense, woven bone. Control implants, consisting only of serum adsorbed into the carrier, showed no bone formation. We conclude that biodegradable gelatin matrix can serve as a carrier for the osteogenic cells of bone marrow in rats in ectopic sites. Such a system may be further developed to augment bone healing.
我们研究了使用可生物降解的猪明胶基质（明胶海绵）作为骨髓细胞的载体，该骨髓细胞可在大鼠的异位部位诱导成骨。从6周龄的Sprague-Dawley大鼠的长骨中获取骨髓细胞，通过酶解使其解离，然后将细胞重悬于大鼠血清中。接着，将0.1 mL血清中的两千万个细胞吸附到1 cm³ 的明胶基质底物片中，并植入活的Sprague-Dawley大鼠的异位部位。在植入后3、4、6和8周取出植入物，进行组织学分析以观察骨和软骨的形成情况。早在3周时就可见到编织骨，并且一直持续到8周。未观察到软骨。破骨细胞最早在3周时出现，4周时数量达到峰值。到6周和8周时，仅残留少量被致密编织骨包围的基质小岛。仅由吸附在载体上的血清组成的对照植入物未显示骨形成。我们得出结论，可生物降解的明胶基质可作为大鼠骨髓成骨细胞在异位部位的载体。这样的系统可能会进一步开发以促进骨愈合。
