中国南方鼻咽癌中13号和14号染色体长臂高频杂合性缺失
High frequency loss of heterozygosity on the long arms of chromosomes 13 and 14 in nasopharyngeal carcinoma in Southern China.
作者信息
Shao Jianyong, Li Yuhong, Wu Qiuliang, Liang Xiaoman, Yu Xingjuan, Huang Lixi, Hou Jinghui, Huang Xiaoming, Ernberg Ingemar, Hu Li-Fu, Zeng Yixin
机构信息
Cancer Center, Sun Yat-Sen University of Medical Sciences, Guangzhou 510060, China.
出版信息
Chin Med J (Engl). 2002 Apr;115(4):571-5.
OBJECTIVE
To investigate the loss of heterozygosity (LOH) on chromosomal arms 13q and 14q in nasopharyngeal carcinoma (NPC) using 21 microsatellite polymorphic markers and to study whether there is a correlation between LOH and clinicopathologic parameters and/or Epstein-Barr virus (EBV) infection in NPC.
METHODS
Sixty cases of NPC were studied using polymerase chain reaction based microsatellite analysis with genescan and genotyping techniques.
RESULTS
LOH was detected on 13q in 78% of NPC tumors, high frequency LOH loci (more than 30%) clustered to 13q12.3-q14.3 and 13q32. On chromosome 14q, LOH was detected in 80% of NPC tumors; high frequency LOH loci clustered to 14q11-q13, 14q21-q24 and 14q32. High frequency LOH at 13q31-q32 correlated with a lower level of EBV infection; LOH on chromosome 14q was closely associated with poor differentiation of NPC tumor cells.
CONCLUSION
Our results suggest that in NPC, LOH on chromosome 13q and 14q are common genetic events, and putative tumor suppressor genes (TSG) residing in these regions may be involved in tumorigenesis.
目的
使用21个微卫星多态性标记物研究鼻咽癌（NPC）中13号和14号染色体臂杂合性缺失（LOH）情况，并探讨LOH与NPC临床病理参数和/或EB病毒（EBV）感染之间是否存在相关性。
方法
采用基于聚合酶链反应的微卫星分析以及基因扫描和基因分型技术对60例NPC进行研究。
结果
78%的NPC肿瘤检测到13号染色体臂LOH，高频LOH位点（超过30%）集中在13q12.3-q14.3和13q32。在14号染色体臂上，80%的NPC肿瘤检测到LOH；高频LOH位点集中在14q11-q13、14q21-q24和14q32。13q31-q32高频LOH与较低水平的EBV感染相关；14号染色体臂LOH与NPC肿瘤细胞低分化密切相关。
结论
我们的结果表明，在NPC中，13号和14号染色体臂LOH是常见的遗传事件，位于这些区域的假定肿瘤抑制基因（TSG）可能参与肿瘤发生。
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