Liu Qun, Zhu Guijin
Department of Obstetrics and Gynecology, Tongji Hospital, Tongji Medical College, Huazhong Science and Technology University, Wuhan 430030, China.
Zhonghua Fu Chan Ke Za Zhi. 2002 May;37(5):274-7.
To evaluate the effects of biopsy methods, biopsy timing and the number of cell removed on in vitro development of embryos.
One hundred and fifty four embryos of good morphology from in vitro fertilization patients were studied. Sixty-six embryos were allocated to the following three groups: chemical drilling biopsy group (26), mechanical drilling biopsy group (26) and control group (20). One cell was removed from the embryos of the two biopsy groups. The remaining 88 embryos were allocated to two groups: biopsy group (44) and control group (44). Two cells were removed from biopsy group by chemical drilling method. The stage of the embryo before biopsy, biopsy time, lysed blastomere, growth potential and hatching capacity of the biopsied embryos, total cell number at the blastocyst stage were recorded and evaluated.
The mean time of biopsy in the chemical drilling group (231 +/- 20) seconds was significantly shorter than that in the mechanical drilling group (262 +/- 23) seconds (P < 0.01). The proportion of embryo developing to blastocyst stage was higher in chemical drilling group as compared with the mechanical group (65% versus 35%, P < 0.05). The total cell number at the blastocyst stage was fewer than those in the 7 to 8-cell embryo and >/= 9-cell embryo groups in the 6-cell embryo group (44 +/- 4 versus 49 +/- 5, 50 +/- 6; P < 0.05). At 9- to 10-cell stage, the proportion developing to the blastocyst stage was reduced in compacted embryos (20%) compared with control group (67%, P < 0.05) also more lysed blastomeres after biopsy were found in compacted embryos (50%) compared with uncompacted embryos (17%). The growth capacity to the blastocyst stage and the total cell number of the blastocyst were not different between one cell removal group and two cells removal group (P > 0.05). However, the proportion developing to the blastocyst stage was reduced after the removal of two cells from the 6-cell stage in comparison to the control (1/8 versus 5/8, P < 0.05).
Compared with mechanical drilling biopsy, chemical drilling technique takes shorter timer and is safer. The suitable biopsy timing was >/= 7-cell stage before embryo compacting. The removal of two cells from >/= 7-cell would not impair in vitro development of embryos.
评估活检方法、活检时机及去除细胞数量对胚胎体外发育的影响。
研究了154例体外受精患者形态良好的胚胎。66个胚胎被分为以下三组：化学打孔活检组（26个）、机械打孔活检组（26个）和对照组（20个）。从两个活检组的胚胎中去除1个细胞。其余88个胚胎被分为两组：活检组（44个）和对照组（44个）。通过化学打孔法从活检组中去除2个细胞。记录并评估活检前胚胎的阶段、活检时间、裂解的卵裂球、活检后胚胎的生长潜能和孵化能力、囊胚阶段的总细胞数。
化学打孔组的平均活检时间（231±20）秒明显短于机械打孔组（262±23）秒（P<0.01）。与机械组相比，化学打孔组胚胎发育至囊胚阶段的比例更高（65%对35%，P<0.05）。6细胞胚胎组囊胚阶段的总细胞数少于7至8细胞胚胎组和≥9细胞胚胎组（44±4对49±5、50±6；P<0.05）。在9至10细胞阶段，致密化胚胎发育至囊胚阶段的比例（20%）低于对照组（67%，P<0.05），并且与非致密化胚胎（17%）相比，致密化胚胎活检后发现更多裂解的卵裂球（50%）。去除1个细胞组和去除2个细胞组之间发育至囊胚阶段的能力和囊胚的总细胞数没有差异（P>0.05）。然而，与对照组相比，从6细胞阶段去除2个细胞后发育至囊胚阶段的比例降低（1/8对5/8，P<0.05）。
与机械打孔活检相比，化学打孔技术所需时间更短且更安全。合适的活检时机是胚胎致密化前≥7细胞阶段。从≥7细胞阶段去除2个细胞不会损害胚胎的体外发育。
