Luo Hong, Mi Mantian, Zhang Qianyong
Department of High Altitude Hygiene, Third Military Medical University.
Zhonghua Xue Ye Xue Za Zhi. 2002 Apr;23(4):176-8.
To study the effect of combination of eicosapentaenoic acid (EPA) and retinoic acid (RA) on the proliferation and differentiation of HL-60 cells and its mechanisms.
MTT was used for cell proliferation analysis, NBT reduction experiment for cell differentiation, reverse transcriptase polymerase chain reaction (RT-PCR) for retinoblastoma (RB) mRNA expression, and Western blot for RB protein (PRB) expression.
The proliferation inhibition rates were 35.74%, 24.38% and 42.75% for RA, EPA and combination of EPA and RA. NBT reduction experiments showed that the differentiation induced by EPA and RA was 5.9 times, and by RA was 2.6 times the capacity of the control. The RB mRNA and PRB expression were not changed by EPA, but significantly decreased by the combination of EPA and RA. Moreover, the dephosphorylation rate of PRB was increased by the treatment with EPA or/and RA.
The changes of RB expression and PRB phosphorylation may be one of the mechanisms of the synergistic effects of EPA and RA on the HL-60 cell proliferation and differentiation.
研究二十碳五烯酸（EPA）与视黄酸（RA）联合应用对HL-60细胞增殖和分化的影响及其机制。
采用MTT法进行细胞增殖分析，硝基蓝四氮唑还原实验检测细胞分化，逆转录聚合酶链反应（RT-PCR）检测视网膜母细胞瘤（RB）mRNA表达，蛋白质免疫印迹法检测RB蛋白（PRB）表达。
RA、EPA及EPA与RA联合应用的增殖抑制率分别为35.74%、24.38%和42.75%。硝基蓝四氮唑还原实验显示，EPA和RA诱导的分化能力分别是对照组的5.9倍和2.6倍。EPA对RB mRNA和PRB表达无影响，但EPA与RA联合应用可使其显著降低。此外，EPA或/和RA处理可提高PRB的去磷酸化率。
RB表达和PRB磷酸化的变化可能是EPA和RA协同影响HL-60细胞增殖和分化的机制之一。
