Alliin lyase (alliinase) from garlic (Allium sativum): crystallization and preliminary X-ray characterization.

作者信息

Shimon Linda J W, Rabinkov Aharon, Miron Talia, Mirelman David, Wilchek Meir, Frolow Felix

机构信息

Department of Chemical Services, The Weizmann Institute of Science, Rehovot 76100, Israel.

出版信息

Acta Crystallogr D Biol Crystallogr. 2002 Aug;58(Pt 8):1335-7. doi: 10.1107/s0907444902010466. Epub 2002 Jul 20.

The enzyme alliinase has been isolated from garlic bulbs and crystallized. The crystals belong to space group P2(1), with unit-cell parameters a = 70.191, b = 127.006, c = 108.085 A, beta = 93.384 degrees. They diffract to 2.2 A at liquid-nitrogen temperature. Analysis of the Patterson self-rotation function suggests that the crystals contain two dimeric molecules per asymmetric unit.