Kraus Ina, Scheffczik Hanno, Eickmann Markus, Kiermayer Simone, Stubbs Milton T, Garten Wolfgang
Institut für Virologie, Robert-Koch-Strasse 17, 35037 Marburg, Germany.
Acta Crystallogr D Biol Crystallogr. 2002 Aug;58(Pt 8):1371-3. doi: 10.1107/s0907444902010132. Epub 2002 Jul 20.
The matrix protein M of Borna disease virus (BDV) is associated with the inner viral membrane and is thought to be a mediator between the nucleocapsid and the lipid-containing envelope in stabilizing the virus shape. The full-length BDV-M gene encoding a 16 kDa protein was expressed in Escherichia coli. M was purified to homogeneity and crystallized by the sitting-drop vapour-diffusion method. The crystals of M belong to the space group I432, with unit-cell parameters a = b = c = 144.6 A, and diffract to 3.1 A.
博尔纳病病毒（BDV）的基质蛋白M与病毒内膜相关，被认为是核衣壳与含脂包膜之间的介质，在稳定病毒形态方面发挥作用。编码16 kDa蛋白的全长BDV-M基因在大肠杆菌中表达。M蛋白被纯化至同质，并通过坐滴气相扩散法结晶。M蛋白的晶体属于空间群I432，晶胞参数a = b = c = 144.6 Å，衍射分辨率为3.1 Å。
