Wei Tao-Tao, Chen Chang, Huang Yong-Jin, Zhao Bao-Lu, Hou Jing-Wu, Xin Wen-Juan, Mori Akitane
Institute of Biophysics, the Chinese Academy of Sciences, Beijing 100101, China.
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 1999;31(3):239-244.
The cytotoxic effect of peroxynitrite on cerebellar granule neurons was studied. Exposure of cerebellar granule neurons to 10 &mgr;mol/L peroxynitrite triggered apoptosis in vitro, which was confirmed by both morphological (nuclear morphology observed by fluorescence microscopy) and biochemical evidence (DNA fragmentation detected by ELISA). Using ESR spin labeling technique, the alteration of biophysical characteristics of neuronal cell membrane during the apoptotic process was studied. The results indicate that after treatment with peroxynitrite, the fluidity of both the surface layer and the deep layer of the neuronal cell membrane decreased markedly, and the S/W ratio of the membrane protein thiol groups increased significantly. Pre-treating cerebellar granule neurons with antioxidant EPC-K1, a novel water-soluble derivative of vitamin C and vitamin E, alleviated the oxidative injury and prevented cells from apoptosis.
研究了过氧亚硝酸盐对小脑颗粒神经元的细胞毒性作用。将小脑颗粒神经元暴露于10μmol/L过氧亚硝酸盐中可在体外引发细胞凋亡，这通过形态学（荧光显微镜观察核形态）和生化证据（ELISA检测DNA片段化）得以证实。使用电子自旋共振自旋标记技术，研究了凋亡过程中神经元细胞膜生物物理特性的改变。结果表明，用过氧亚硝酸盐处理后，神经元细胞膜表层和深层的流动性均显著降低，膜蛋白硫醇基团的S/W比显著增加。用抗氧化剂EPC-K1（一种新型的维生素C和维生素E水溶性衍生物）预处理小脑颗粒神经元，可减轻氧化损伤并防止细胞凋亡。
