Rodríguez Ana M, Roca Pilar, Palou Andreu
Departament de Biologia Fonamental i Ciències de la Salut, Laboratori de Biologia Molecular, Nutrició i Biotecnologia, Universitat de les Illes Balears, Palma de Mallorca, 07071, Spain.
Pflugers Arch. 2002 Jul;444(4):484-90. doi: 10.1007/s00424-002-0829-6. Epub 2002 Apr 17.
The effect of cold exposure, being overweight and their interaction was investigated on the response of uncoupling proteins UCP1, UCP2 and UCP3 and the alpha(2)/beta(3) adrenergic receptor (AR) balance in brown adipose tissue (BAT), as well as the involvement of leptin gene expression in white adipose tissues, in control and overweight male rats of the dietary obesity model known as the post-cafeteria model. UCP1, UCP2 and UCP3 mRNAs were up-regulated by cold, with a synergic effect of cold exposure and being overweight on UCP1 mRNA levels (with the related UCP1 protein response), and with UCP2 mRNA showing a parallel response. Furthermore, the BAT alpha(2)/beta(3) AR ratio was diminished in overweight rats. The results suggest that the UCP1-dependent thermogenic capacity in BAT of post-cafeteria overweight rats has a more sensitive response to cold exposure and that UCP2 and UCP3 could be somehow involved in the thermogenic response but differentially regulated. Moreover, the diminished alpha(2)/beta(3) AR ratio in BAT could be one of the factors involved in the more sensitive response of overweight rats to cold in terms of BAT thermogenesis-related parameters.
在饮食性肥胖模型（即后自助餐厅模型）的对照和超重雄性大鼠中，研究了冷暴露、超重及其相互作用对棕色脂肪组织（BAT）中解偶联蛋白UCP1、UCP2和UCP3以及α(2)/β(3)肾上腺素能受体（AR）平衡的反应的影响，以及瘦素基因表达在白色脂肪组织中的参与情况。UCP1、UCP2和UCP3的mRNA受寒冷上调，冷暴露和超重对UCP1 mRNA水平（以及相关的UCP1蛋白反应）有协同作用，UCP2 mRNA表现出平行反应。此外，超重大鼠BAT中的α(2)/β(3) AR比值降低。结果表明，后自助餐厅超重大鼠BAT中依赖UCP1的产热能力对冷暴露有更敏感的反应，并且UCP2和UCP3可能以某种方式参与产热反应但调节方式不同。此外，BAT中α(2)/β(3) AR比值降低可能是超重大鼠在BAT产热相关参数方面对寒冷更敏感反应的因素之一。
