Miño de Kaspar Herminia, Engelbert Michael, Thiel Martin, Grasbon Thomas, Ta Christopher N, Klauss Volker, Kampik Anselm
Department of Ophthalmology, Stanford University, School of Medicine, California, USA.
Graefes Arch Clin Exp Ophthalmol. 2002 Jul;240(7):557-64. doi: 10.1007/s00417-002-0490-y. Epub 2002 Jun 20.
To determine the effect of prophylactic intravenous imipenem on experimental Staphylococcus aureusendophthalmitis.
The right eyes of 36 New Zealand albino rabbits received an intraocular injection of 100, 1,000, or 10,000 colony-forming units (CFU) of S. aureus. Each of these three groups was subdivided into a control group (no antibiotic prophylaxis) and a prophylactic group, which received 37.5 mg/kg imipenem 8 h prior to inoculation of bacteria, 30 min prior to inoculation of bacteria, and 8 h after inoculation of bacteria. Eyes were observed daily by slit-lamp examination and funduscopy. The eyes were enucleated after 5 and 9 days for the control and prophylactic groups, respectively. Vitreous samples were cultured for bacteria, and the eyes were prepared for histologic evaluation.
All eyes that received imipenem demonstrated significantly less inflammation (two-way analysis of variance; P<0.001) on clinical examinations than did control eyes. The prophylactic groups that had received 100 CFU and 1,000 CFU of bacteria also had significantly fewer positive vitreous cultures than the corresponding controls (chi(2) test; P<0.001 and P<0.01, respectively). Histologically, the prophylactic groups injected with 100 CFU and 1,000 CFU of bacteria had significantly less inflammation than the control groups (Student's t-test; P<0.00001 and P<0.0001, respectively). There was no significant difference in the number of positive vitreous cultures or histologically between the control and prophylactic groups that received 10,000 CFU of bacteria.
Prophylactic intravenous imipenem can prevent or significantly reduce the severity of experimental endophthalmitis.
确定预防性静脉注射亚胺培南对实验性金黄色葡萄球菌性眼内炎的影响。
36只新西兰白兔的右眼接受了100、1000或10000个金黄色葡萄球菌菌落形成单位（CFU）的眼内注射。这三组中的每组又分为对照组（不进行抗生素预防）和预防组，预防组在接种细菌前8小时、接种细菌前30分钟以及接种细菌后8小时接受37.5mg/kg亚胺培南。每天通过裂隙灯检查和眼底镜检查观察眼睛。对照组和预防组分别在5天和9天后摘除眼球。对玻璃体样本进行细菌培养，并对眼睛进行组织学评估。
所有接受亚胺培南治疗的眼睛在临床检查中显示出的炎症明显少于对照眼（双向方差分析；P<0.001）。接受100CFU和1000CFU细菌的预防组玻璃体培养阳性的数量也明显少于相应的对照组（卡方检验；分别为P<0.001和P<0.01）。组织学上，注射100CFU和1000CFU细菌的预防组炎症明显少于对照组（学生t检验；分别为P<0.00001和P<0.0001）。接受10000CFU细菌的对照组和预防组之间玻璃体培养阳性数量或组织学上没有显著差异。
预防性静脉注射亚胺培南可预防或显著降低实验性眼内炎的严重程度。
