Rüegg U T, Gattner H G
Hoppe Seylers Z Physiol Chem. 1975 Oct;356(10):1527-33. doi: 10.1515/bchm2.1975.356.2.1527.
All 4 S-sulpho groups of the S-sulpho substituted insulin A-chain could be removed with 4 mol of tributylphosphine. Reduction of a 1:1 mixture of both S-sulpho insulin chains with tributylphosphine followed by air oxidation gave insulin which was isolated in pure form and high yield. Removal of excess reducing agent was not necessary, in contrast to the usual procedures employing thiols for the reduction step. This constitutes a rapid and simple method for the generation of insulin from its chains. A new method for the purification of S-sulpho-A-chain has been developed.
S-磺基取代胰岛素A链的所有4个S-磺基可被4摩尔三丁基膦去除。用三丁基膦还原两种S-磺基胰岛素链的1:1混合物，然后进行空气氧化，得到的胰岛素以纯形式且高产率分离出来。与通常在还原步骤中使用硫醇的程序不同，无需去除过量的还原剂。这构成了一种从其链生成胰岛素的快速简便方法。已开发出一种纯化S-磺基-A链的新方法。
