人肽激素血管紧张素II结构基因的全合成
Total synthesis of a structural gene for the human peptide hormone angiotensin II.
作者信息
Köster H, Blöcker H, Frank R, Geussenhainer S, Kaiser W
出版信息
Hoppe Seylers Z Physiol Chem. 1975 Oct;356(10):1585-93. doi: 10.1515/bchm2.1975.356.2.1585.
PMID:1213674
Abstract
Seven oligonucleotide chains containing between 6 and 11 nucleotide units were synthesized. The segments were phosphorylated by T4 polynucleotide 5'-hydroxyl-kinase and joined by T4 polynucleotide synthetase (ATP) to give the double-stranded DNA consisting of 33 base pairs. The DNA sequence was deduced from the known peptide sequence according to the genetic code.
摘要
合成了7条含有6至11个核苷酸单元的寡核苷酸链。这些片段通过T4多核苷酸5'-羟基激酶进行磷酸化，并通过T4多核苷酸合成酶（ATP）连接，以得到由33个碱基对组成的双链DNA。根据遗传密码从已知的肽序列推导DNA序列。
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