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人和猪生长激素的免疫学特性。应用于猪血清中生长激素的测定。

Immunological identity of human and porcine growth hormones. Application to determination of growth hormone in porcine serum.

作者信息

Etzrodt H, Schleyer M, Voigt K H, Pfeiffer E F

出版信息

Hoppe Seylers Z Physiol Chem. 1975 Dec;356(12):1839-41. doi: 10.1515/bchm2.1975.356.2.1839.

DOI:10.1515/bchm2.1975.356.2.1839
PMID:1213679
Abstract

In two radioimmunoassay systems with iodinated human growth hormone as tracer and anti-human growth hormone or anti-porcine growth hormone as binding site, the standard curves for human and for porcine growth hormone were parallel. In both systems the porcine growth hormone preparation had to be added in about the same excess as compared to the human hormone. It was concluded that the human and the porcine hormones behave in an identical way and that the values obtained in radioimmunoassay for human growth hormone represent the amount of immunologically active molecules in the porcine growth hormone preparation. As parallel standard curves were obtained with porcine serum, the concentration of growth hormone is porcine serum may be determined by radioimmunoassay for human growth hormone.

摘要

在两个以碘化人生长激素为示踪剂、以抗人生长激素或抗猪生长激素为结合位点的放射免疫分析系统中,人生长激素和猪生长激素的标准曲线是平行的。在这两个系统中,与人生长激素相比,猪生长激素制剂必须以大致相同的过量添加。得出的结论是,人和猪的激素表现方式相同,并且放射免疫分析中获得的人生长激素值代表了猪生长激素制剂中免疫活性分子的量。由于用猪血清获得了平行的标准曲线,因此猪血清中生长激素的浓度可以通过放射免疫分析人生长激素来测定。

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