Brown C R, Hartree E F
Hoppe Seylers Z Physiol Chem. 1975 Dec;356(12):1909-13. doi: 10.1515/bchm2.1975.356.2.1909.
Ram seminal plasma, and ejaculated ram spermatozoa that have been washed with 0.25M sucrose, both contain acrosin inhibitor. The aim of this work was to determine whether the intracellular inhibitor originates from the seminal plasma. The amounts of inhibitor in ejaculated and epididymal spermatozoa were measured and compared with the amounts present in the seminal plasma of normal and vasectomized rams. One ejaculated ram spermatozoon contained 2.1 amol (2.1 X 10(-18) mol) of inhibitor and one epididymal spermatozoon contained 3.3 amol of inhibitor. (All molarities are mean values based on pooled ram semen or on single ejaculates from three vasectomized rams.) Calculations from results in earlier publications indicated that one ejaculated ram spermatozoon contains about 3 amol of acrosin; thus the inhibitor: acrosin ratio in washed ram spermatozoa is approximately 1. One ml of ram semen contains, on average, 3 X 10(9) spermatozoa and not more than 0.8 ml of seminal plasma. This number of ejaculated spermatozoa would contain 6.3 nmol of inhibitor, while the same number of epididymal spermatozoa would contain 9.9 nmol of inhibitor. These values exceed the quantities of inhibitor present in 0.8 ml of normal seminal plasma (approximately 1.6 nmol) or in 0.8 ml of seminal plasma from vasectomized rams (approximately 2.3 nmol). We conclude that seminal plasma is not a major source of the acrosin inhibitor that can be recovered from washed ejaculated ram spermatozoa.
公羊精液和用0.25M蔗糖洗涤过的射出公羊精子均含有顶体蛋白酶抑制剂。本研究的目的是确定细胞内抑制剂是否源自精液。测定了射出精子和附睾精子中的抑制剂含量，并与正常公羊和输精管结扎公羊精液中的抑制剂含量进行比较。一个射出的公羊精子含有2.1 amol（2.1×10⁻¹⁸mol）抑制剂，一个附睾精子含有3.3 amol抑制剂。（所有摩尔浓度均为基于合并的公羊精液或三只输精管结扎公羊的单次射精的平均值。）根据早期出版物的结果计算表明，一个射出的公羊精子含有约3 amol顶体蛋白酶；因此，洗涤过的公羊精子中抑制剂与顶体蛋白酶的比例约为1。1毫升公羊精液平均含有3×10⁹个精子，精液不超过0.8毫升。这个数量的射出精子将含有6.3 nmol抑制剂，而相同数量的附睾精子将含有9.9 nmol抑制剂。这些值超过了0.8毫升正常精液（约1.6 nmol）或0.8毫升输精管结扎公羊精液（约2.3 nmol）中所含抑制剂的量。我们得出结论，精液不是可从洗涤过的射出公羊精子中回收的顶体蛋白酶抑制剂的主要来源。
